Order number: M6360.0500Shipping: shipped at RT, store at +2°C to +8°C.
Delivery time: 3- 8 working days
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AEBSF is an irreversible inhibitor of Thrombin and other Serin proteases (Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin) by sulfonylation of a functional group in the active centre of the enzyme. AEBSF is much less toxic compared to PMSF and DFP and therefore recommended to substitute these reagents. AEBSF is highly soluble in water resulting in a solution of pH 6.5. At this pH AEBSF is most stable. We recommend to store stock solutions (20 mM or 100 mM in water or buffer) at -20°C for up to 2 months and prepare fresh working solutions. AEBSF is used at a working concentration of 0.1 - 0.2 mM. Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-585, Nathan, D.F. & Linquist, S. (1995) Mol. Cell. Biol., 15, 3917-3925.
Assay: min. 98.0% (HPLC)
Solubility (H2O, 20°C): 50g/L
MP = 181° - 185°C
Loss on drying: max. 1%
MW = 239.69 g/mol
most stable at pH around 6.5. Stable for 1 week at +2°C to +8°C.
Stock solution stable for 2 months at -20°C.
Sicherheits Hinweise / SafetyH-Sätze: H314
Klassifizierungen / ClassificationCAS-Nr: 30827-99-7
Dokumente - Protokolle - Downloads
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