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Aprotinin is a serin protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Effective concentration equimolar to protease, normally 2-10µg/mL (about 1mM). Aprotinin is stable for long time at 4°C as lyophilised powder. It is also possible to store stock solutions for several years at -20°C. We recommend to prepare small samples as stock solutions, as repetitive freeze-thawing cycles as aggregation will occur after few thawing and freezing cycles.
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
purified inhibitor of Trypsin, Chymotrypsin, Plasmin and especially Kallikrein
Assay: min. 90.0 % (protein assay)
Acitivity: min. 5250 KIU/mg (>5.17 TIU/mg)
pH (1%, H2O) = 5.0 - 7.5
Water (K.F.): max. 6%
MW = 6511.52 g/mol.
activity conversions: >5250 KIU/mg; >5.17 TIU/mg; >2.58 PEU/mg
application:for in vitro inhibition of fibrinolytic activity in blood samples
Unit Definition:1 IU (inhibitor unit) inhibits 1 U trypsin as defined by cleavage of 1µmol BAEE (N-benzoyl-L-arginine ethyl ester) per minute. Activity in other units: min. 3 Ph. Eur. Units (PEU)/mg, based on dried substance. Unit definition: 1 Ph. Eur. Unit of aprotinin inhibits 50 % of the enzymatic activity of 2 microkatals trypsin, measured with BAEE as substrate at pH8.0 and 25°C. Activity in other units: min. 5850 Kallikrein Inactivator Units (KIU)/mg, based on dried substance. (KIU = PEU x 60 x 32.5).
Sicherheits Hinweise / SafetyH-Sätze: H317, H334
GHS-Symbole: GHS07, GHS08
Klassifizierungen / ClassificationEC-Nr: 232-994-9
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Aprotinin from bovine lung. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.