DL-Dithiothreitol (DTT) - BioChemica

DL-Dithiothreitol


Order number: M6031.0005

Shipping: shipped on wet ice, store at +2°C to +8°C
CAS-Nr: 3483-12-3

Delivery time: 3- 8 working days
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€45.00 *

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Dithiothreitol (DTT) is an isomer (epimer) of dithioerythritol (DTE). In principle, DTT can be... more
Product information "DL-Dithiothreitol (DTT) - BioChemica"

Dithiothreitol (DTT) is an isomer (epimer) of dithioerythritol (DTE). In principle, DTT can be exchanged for DTE or DTE for DTT, of which both are excellent reducing substances.

Dithiothreitol (DTT), like ß-mercaptoethanol, is used as a reducing agent to increase the stability of proteins as protection against the undesired oxidation of cysteine residues. Proteins can oxidize during gel electrophoresis. This results in broader bands (reduced resolution) and non-reproducible mobility (incorrect assessment of the molecular weight).

DTT can be used in practically all experiments in a three to four times lower molar concentration than ß-mercaptoethanol. It is less toxic, less odor intensive and does not form mixed disulfides like ß-mercaptoethanol. DTT is water-soluble and is usually prepared as a 1M stock solution. This solution should be stored in aliquots at -20°C and protected from heat while working. A sufficient amount should be added to the solutions in the experiment, since it oxidizes relatively quickly in air.

The working concentration is between 0.1 and 1mM, but must be increased to up to 5mM, for example in the production of plant extracts, or up to 10mM in the 'large scale in situ isolation' of proteins during fermentation. While DTT has a pK value of 8.3 and 9.5, the pK is 9.0 and 9.9 for DTE. Since both compounds show their reducing properties only in the ionized state, their reducing effect is greatest in the pH range 7.0 - 9.5. In the lower pH range, DTT is more active than DTE. The reducing effect can be stopped by acidification (e.g. acetic acid to pH3).

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Our comment on "DL-Dithiothreitol (DTT) - BioChemica"
very hygroscopic
Specifications: Purity: min. 99% (iodom.) Appearance: white to off-white powder MP = 40-44°C... more
 

Technical Data:

Specifications:
Purity: min. 99% (iodom.)
Appearance: white to off-white powder
MP = 40-44°C
pH (0.1M in water): 4.0-6.0
DTT (oxidised): max. 0.5%
Water: max. 0.5%
C4H10O2S2
MW = 154.3 g/mol

Product is very hygroscopic! My clump while stored. Product packed under inert gas. Please open only under inert gas atmosphere.

application:

used in stead of mercaptoethanol to reduce disulfide bonds of proteins.

Source

synthetic from

Sicherheits Hinweise / Safety

H-Sätze: H302, H315, H319
GHS-Symbole: GHS07
R-Sätze: R22-36/38
Gefahrstoffkürzel: Xn

Klassifizierungen / Classification

EC-Nr: 222-468-7
CAS-Nr: 3483-12-3
eclass-Nr: 32-16-04-01
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Here you will find information and further literature on DL-Dithiothreitol (DTT) - BioChemica. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.