Agarose Tiny - low melting, high resolution
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Agarose Tiny is a low-melting high resolution agarose that is suitable for molecular biology applications and produces gels with better separation properties and greater clarity than standard agarose. It is very transparent even at high gel concentrations (3.0 - 4.5%) and can be used with all buffer systems. The low melting temperature makes Agarose Tiny ideal for preparative DNA and RNA electrophoresis, while the low gelling temperature is ideal for tissue culture cell cloning experiments and viral plaque assays.
In addition to the above mentioned properties, Agarose Tiny can also be used for high-resolution (2 bp) gel electrophoresis in the range from 10 to 500 bp. Example: PCR products or small DNA fragments after restriction digestion or in mutation analyses.
Like Lonza's SeaPlaque™, Agarose Tiny has a low melting point (<64°C, 3.0% gel) and gel point (<25°C, 3.0% gel) and low DNA-binding capacity. Their use is particularly recommended for preparative RNA and DNA gels.
Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.
Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054) > available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.
Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase (S5223) >, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.
High resolution agarose (normal melting or low melting) for high resolution elektrophoresis
to differentiate DNA fragments with only 2 bp size difference: M3046 (Agarose Tiny (low melt, high resolution comparable to NuSieve®) >, M3048 Agarose Tiny LMH > (normal melting point, high resolution) or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).
For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye GelRed® (M3199) > is recommended.
Test sample available! No shipping costs within Germany.
low EEO low melting low gel strength high resolution of 2bp difference in DNA size very clear and transparent gel
Electroendosmosis (EEO): <0.12
Gel strength (1.5%): >200 g/cm2
Gel strength (3.0%): >300 g/cm2
Gelling temperature (3.0%): <30°C
Melting temperature (1.5%): <72°C
pH (1% in water): 5 - 7
Loss on drying: max. 7%
Ash: max. 0.7%
DNAse and RNAse: not detected.
application:Cloning of tissue culture cells, Viral plaque assays, DNA/RNA preparative electrophoresis.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 232-731-8
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Agarose Tiny - low melting, high resolution. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Dissection of the Complex Phenotype in Cuticular Mutants of Arabidopsis Reveals a Role of SERRATE as a Mediator
Derry Voisin, Christiane Nawrath, Sergey Kurdyukov, Rochus B. Franke, José J. Reina-Pinto, Nadia Efremova, Isa Will, Lukas Schreiber, Alexander Yephremov
PLoS Genet. 2009 Oct; 5(10): e1000703. Published online 2009 Oct 30. doi: 10.1371/journal.pgen.1000703