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High resolution (2 bp), low melting agarose for fine resolution of small DNA fragments in the 10 bp to 500 bp range. It can be used for genotyping, allele sizing and short tandem repeat analysis. Agarose Tiny works as a molecular screen and has twice the resolution of the finest sieving agaroses. It can discriminate between fragments which differ by only 2-4 bp in length and thus, is able to compete with acrylamide gels, being much easier to handle as the latter. Agarose Tiny 2% to 4% gels give similar results to Polyacrylamide 6% to 8% gels. For efficient resolution, the concentration of Agarose Tiny should be adjusted according to the range of band sizes analyzed, and the gel running buffer used (TAE or TBE).
Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.
Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054) > available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.
Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase (S5223) >, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.
High resolution agarose (normal melting or low melting) for high resolution elektrophoresis
to differentiate DNA fragments with only 2 bp size difference: M3046 (Agarose Tiny (low melt, high resolution comparable to NuSieve®) >, M3048 Agarose Tiny LMH > (normal melting point, high resolution) or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).
For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye GelRed® (M3199) > is recommended.
Test sample available! No shipping costs within Germany.
low melting low gel strength high resolution of 2bp difference in DNA size
Electroendosmosis (EEO): <0.12
Gel strength (1.5%): >200 g/cm2
Gel strength (3.0%): >300 g/cm2
Gelling temperature (3.0%): <30°C
Melting temperature (1.5%): <72°C
pH (1% in water): 5 - 7
Loss on drying: max. 7%
Ash: max. 0.7%
DNAse and RNAse: not detected.
application:for electrophoresis of small DNA fragments.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 232-731-8
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Agarose Tiny - low melting, high resolution. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Derry Voisin, Christiane Nawrath, Sergey Kurdyukov, Rochus B. Franke, José J. Reina-Pinto, Nadia Efremova, Isa Will, Lukas Schreiber, Alexander Yephremov
PLoS Genet. 2009 Oct; 5(10): e1000703. Published online 2009 Oct 30. doi: 10.1371/journal.pgen.1000703