Order number: M3051.0025Shipping: shipped and stored at RT
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Agarose Mega resolved DNA and RNA fragments from >100bp up to 50kb. Due to the high gel strength the agarose is ideally suited for Southern- and Northern blotting experiments or Pulsed Field Gel Electrophoresis (PFGE) as gel will not break during handling or electrophoresis. Due to the high gel strength, low concentrations of agarose may be used and high molecular weight molecules migrate rapidly without significant resctriction. The high gel strength reduces separation times for large DNA molecules while maintaining high resolution. Agarose Mega can also be used for the preparative separation of nucleic acids or for Ouchterlony or radial immundiffusion experiments.
Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.
Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.
Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase (S5223) >, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.
High resolution agarose (normal melting or low melting) for high resolution elektrophoresis
to differentiate DNA fragments with only 2 bp size difference: M3046 (Agarose Tiny (low melt, high resolution comparable to NuSieve®) >, M3048 Agarose Tiny LMH > (normal melting point, high resolution) or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).
For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye GelRed® (M3199) > is recommended.
Agarose for separation of proteins and genomic DNA (PFGE)
Electroendosmosis (EEO): <0.12
Gel strength (1%): 1800 g/cm2
Gel strength (1.5%): >3200 g/cm2
Gelling temperature: 36°C (± 1.5°C)
Melting temperature >88°C (± 1.0°C)
pH (1% in water): 6.0
Loss on drying: max. 7%
Ash: max. 0.25%
DNAse and RNAse: not detected
application:for PFGE electrophoresis (pulsed field gel electrophoresis)
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 232-731-8
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Agarose Mega. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.