Lambda-DNA (undigested)

Lambda-DNA (undigested)


Order number: M3073.0500

Shipping: Shipment: on wet ice. Store at -20°C. For laboratory usage only!

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Restriction enzyme-digested lambda DNA (48,502bp) generates molecular weight size markers... more
Product information "Lambda-DNA (undigested)"

Restriction enzyme-digested lambda DNA (48,502bp) generates molecular weight size markers routinely used in gel analysis of nucleic acids. The complete nucleotide sequence has been determined. Lit.: Sanger, F. et al. (1982) J. Mol. Biol. 162, 729.

Details about the DNA sequence can be found in the data sheet attachement.

Description/Preparation:

The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). The molecular weight is 31.5 x 10E6 daltons and is 48,502 base pairs in length.
The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration. The DNA is isolated from the purified phage by phenol/chloroform extraction and dialyzed against 10mM Tris-HCl (pH7.4) and 1mM EDTA.

The product is methylated.

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Our comment on "Lambda-DNA (undigested)"
- GenLadders for DNA sizing cover a broad range of DNA molecular weights - Sharp bands, brigth reference bands - Ready-to-use ladders are stable for 6 months at room temperature
Specifications: Concentration: 0.2 to 0.5mg/mL Storage buffer: 10mM Tris/HCl (pH7,4), 10mM... more
 

Technical Data:

Specifications:
Concentration: 0.2 to 0.5mg/mL
Storage buffer: 10mM Tris/HCl (pH7,4), 10mM NaCl, 1mM EDTA.

application:

Can be used for preparation of molecular weight standard for DNA electrophoresis.

Source

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
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Dokumente - Protokolle - Downloads

Here you will find information and further literature on Lambda-DNA (undigested). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
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Dokumente - Protokolle - Downloads

Is the Genaxxon bioscience Lambda DNA methylated?

Lambda DNA from Genaxxon bioscience is partially methylated. However, we have not determined the actual degree of methylation. In general, not all DNA isolated from E.coli is methylated to the same degree. While pBR322 DNA is fully modified, only about 50% of the possible methylation site of lambda DNA are methylated, presumably because the methylase does not have an opportunity to fully methylate the DNA before it is packaged into the phage head.