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Bacteriophage T7 DNA is isolated from an infected E. coli strain. Phage T7 DNA is a linear DNA of 39937 bp. Some restriction enzyme has no recognition sequences on lambda DNA, therefore these enzymes are to be analyzed on other DNA substrate.
T7 DNA is the most popular alternative to Lambda DNA substrate for restriction endonucleases testing. The complete nucleotide sequence has been determined and published. For details see reference.
Dunn, J.J. and Studier, F.W. (1983), Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements, J. Mol. Biol. 166 (4), 477-535.
Storage buffer: 10mM Tris, pH8.0, 10mM NaCl, 1mM EDTA.
application:Can be used for preparation of molecular weight standard for DNA electrophoresis.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-09-01
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