GenLadder 100 bp Plus with gel staining dye
Order number: M3237.0050
Shipping: Shipment: on wet ice. Store at -20°C. For laboratory usage only! Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
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The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow additional DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye. Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. At the same time, it is possible to check whether a PCR was successful or not before applying the PCR sample!
This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens!
The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp.
The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography.
Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL).
The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL.
The recommended amount of DNA marker per lane is 0,5μg (6µL).
GenLadder DNA marker are available in a ready-to-use format (premixed with 6X DNA Loading Dye: M3094 (GenLadder 100 bp Plus ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.
The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
- GenLadders for DNA sizing cover a broad range of DNA molecular weights - Sharp bands, brigth reference bands - Ready-to-use ladders are stable for 6 months at room temperature
Technical Data:
Specifications:
ready-to-use DNA marker containing already loading dye plus a fluorescence gel staining dye for visualization of DNA bands
Number of bands: 12
Fragment sizes: 100 bp, 200 bp, 300 bp, 400 bp, 2x500 bp, 600 bp, 700 bp, 800 bp, 900 bp, 1000 bp, 2x 1500 bp, and 3000 bp.
DNA is dissolved at a concentration of 90µg per 1mL.
Buffer contains orange G & xylene cyanol FF as tracking dyes.
Buffer contains a fluorescent gel staining dye for visualization of DNA bands.
We recommend to load 5-6µL per lane.
application:
Molecular weight standard for DNA electrophoresisSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Here you will find information and further literature on GenLadder 100 bp Plus with gel staining dye. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.