Order number: D2030.1010Shipping: shipped and stored at RT
Delivery time: 3- 8 working days
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Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.89M Tris-borate, 0.02M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.
You will find more ready-to-use buffers here: ready-to-use buffers >
pre-weight ready-to-use certified
Exactly pre-weight powder mix for 1L of 10X TBE buffer
Final concentrations: 0.89M Tris/borate, 0.02M EDTA
pH of 1-time solution: 8.3 +/-0.15 (25°C)
Rnases/Dnases: not detectable.
Sicherheits Hinweise / SafetyH-Sätze: H315, H319, H360D
GHS-Symbole: GHS07, GHS08
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
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Here you will find information and further literature on 10X Tris-Borate-EDTA buffer (pH8.3) - 10 bags. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.