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Dextran sulfate is a heparin-like polysaccharide which increases the hybridization rate of nucleic acids between 3-fold and 100-fold depending on the sample used. This is accomplished by reducing the volume of the DNA-containing solution through the polymer, thereby concentrating the DNA. However, dextran sulfate should only be used if the hybridization rate or the amount of DNA is a limiting factor for the identification of 'rare' sequences. The effect of dextran sulfate is highest for polynucleotides with> 250 nucleotides. No effect is observed for oligonucleotides <14 bases. For the hybridization of bacterial colonies or plaques the application also means no advantage. It can even lead to a high background. The handling of a dextran sulfate solution is additionally made difficult by the high viscosity of the solution. A 50% stock solution is slightly yellowish, the working concentration is 10%. If aqueous dextran sulfate solutions are to be autoclaved, they must, For example with sodium bicarbonate, in order to prevent decomposition during autoclaving.
Polyionic derivative of dextran with an average molecular weight of 500 kDa. White powder; chloride: max. 0.01%; Pb: max. 0.0005%; Ca: max. 0.02%; Fe: max. 0.0002%; solubility (10%, water): clear, colourless; pH (1%, water): 5.0 - 7.5; Sulfate ash: 40-
application:Hybridisation of nucleic acids
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 9011-18-1
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