Order number: M3028.0010Shipping: shipped at RT, must be stored at -20°C
Delivery time: 3- 8 working days
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DNase I is an endonuclease isolated from bovine pancreas that digests double- and single-stranded DNA into oligo- and mono-nucleotides. This DNAse I is not RNase-free. RNAse-free DNase I is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RT-PCR. Since no RNA purification procedure removes 100% of the DNA, RNA samples should be digested with DNase I before RT-PCR. A simple 15 minute digestion at room temperature removes the contaminating DNA. DNase I is inactivated by adding the stop solution and heating. Heating also denatures the RNA, so the RNA can be used directly for reverse transcription. This DNase I is suited for nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to RT-PCR.
Eliminates DNA from RNA samples.
ca. 3000 units/mg
application:removes DNA for RNA purification procedures.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 232-667-0
Dokumente - Protokolle - Downloads
Here you will find information and further literature on DNase I (EC 18.104.22.168). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.