Proteinase K solution (20mg/mL)

Proteinase K solution (20mg/mL)
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Order number: QM3037.0005

Shipping: shipped at RT, stored at -20°C
CAS-Nr: 39450-01-6

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Proteinase K from Tritirachium album belongs to the familiy of subtilisin-like serine proteases.... more
Product information "Proteinase K solution (20mg/mL)"

Proteinase K from Tritirachium album belongs to the familiy of subtilisin-like serine proteases. It has an endo- and exoproteolytic activity. Activated by calcium (1 - 5mM), Proteinase K exhibits a broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents after hydrophobic amino acids. Proteins will be completely digested, if the incubation time is long and the protease concentration high enough.
As Calcium is not directly involved in the catalytic process, removal of the Calcium ions will almost not influence the enzymatic activity but the stability of the enzyme is reduced (about 80% residual activity). The pH-optimum is at 8, but the enzyme is active over a wide pH-range (pH5 - 12). An elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several times, like the addition of 0.5 - 1% SDS. Temperatures above 65°C, trichloroacetic acid or the serine protease inhibitors AEBSF (M6360) >, PMSF (M3194) > or DFP inhibit the activity. For complete denaturation temperatures above 95°C are necessary.
Proteinase K will not be inhibited by  EDTA, urea (1 - 4 M), SDS, citrate, iodoacetic acid or, interestingly, by other serine protease inhibitors like TLCK and TPCK. In case that proteinase K has to be inactivated, make sure, that the temperature is not below 95°C and the time not shorter than 10 minutes. A TCA-precipitation is well suited, too.

Proteinase K is used for the digestion of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases. The digest with Proteinase K for the purification of nucleic acids is performed in the presence of EDTA (inhibition of magnesium-dependent enzymes).

Proteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.

More products around "Proteinase K solution (20mg/mL)"
Specifications: Conc. 20mg/mL proteinase K Activity: min. 600 mAnsonU/mL (>600 units/mL)... more
 

Technical Data:

Specifications:
Conc. 20mg/mL proteinase K
Activity: min. 600 mAnsonU/mL (>600 units/mL)
spec. Activity: >30 mAnsonU/mg (>30 units/mg)

 

application:

Protein digest in DNA samples for example: Purification of genomic DNA from bacteria (miniprep): Bacteria from a saturated liquid culture are lysed and proteins are removed by a digest with 100μg/mL Proteinase K for 1 h at 37°C. Whole-Mount in situ hybridization and determination of RNAs in vertebrate embryos and isolated organs: Digest of the sample with e. g. 10μg/mL Proteinase K for 15 minutes at room temperature. The period of the treatment and/or the concentration of the enzyme has to be optimized. Prepration of DNA from cells or tissue for PCR: Cells or tissue are incubated over night at 50°C with 100μg/mL Proteinase K. Isolation of vaccinia virus DNA: Digest the virus in a suspension with 2mg/mL Proteinase K for 4 h at 37°C.

Unit Definition:

Concentration: 20mg/mL, Activity: >30 U/mg (>600U/mL). 1 unit is definded as the enzyme acitivity which liberates folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 minute at 37°C using haemoglobin as substrate.

Source

Tritirachium album

Sicherheits Hinweise / Safety

H-Sätze: H315, H319, H335
GHS-Symbole: GHS07, GHS08
R-Sätze: R.36/37/38-42
Gefahrstoffkürzel: Xn

Klassifizierungen / Classification

EC-Nr: 254-457-8
CAS-Nr: 39450-01-6
eclass-Nr: 32-16-04-10
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Here you will find information and further literature on Proteinase K solution (20mg/mL). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

Decreased Enhancer-Promoter Proximity Accompanying Enhancer Activation
Nezha S. Benabdallah, Iain Williamson, Robert S. Illingworth, Lauren Kane, Shelagh Boyle, Dipta Sengupta, Graeme R. Grimes, Pierre Therizols, Wendy A. Bickmore
Mol Cell. 2019 Nov 7; 76(3): 473–484.e7. doi: 10.1016/j.molcel.2019.07.038
PMCID: PMC6838673

Glucocorticoid Receptor Binding Induces Rapid and Prolonged Large-Scale Chromatin Decompaction at Multiple Target Loci
Alasdair W. Jubb, Shelagh Boyle, David A. Hume, Wendy A. Bickmore
Cell Rep. 2017 Dec 12; 21(11): 3022–3031. Published online 2017 Dec 12. doi: 10.1016/j.celrep.2017.11.053
PMCID: PMC5745231

Enhancer turnover is associated with a divergent transcriptional response to glucocorticoid in mouse and human macrophages
Alasdair W Jubb, Robert S Young, David A Hume, Wendy A Bickmore
J Immunol. Author manuscript; available in PMC 2016 Jul 15.Published in final edited form as: J Immunol. 2016 Jan 15; 196(2): 813–822. Published online 2015 Dec 9.  doi: 10.4049/jimmunol.1502009
PMCID: PMC4707550

Active promoters give rise to false positive ‘Phantom Peaks’ in ChIP-seq experiments
Dhawal Jain, Sandro Baldi, Angelika Zabel, Tobias Straub, Peter B. Becker
Nucleic Acids Res. 2015 Aug 18; 43(14): 6959–6968.  Published online 2015 Jun 27. doi: 10.1093/nar/gkv637
PMCID: PMC4538825

ISWI Remodelling of Physiological Chromatin Fibres Acetylated at Lysine 16 of Histone H4
Henrike Klinker, Felix Mueller-Planitz, Renliang Yang, Ignasi Forné, Chuan-Fa Liu, Lars Nordenskiöld, Peter B. Becker
PLoS One. 2014; 9(2): e88411.  Published online 2014 Feb 6. doi: 10.1371/journal.pone.0088411
PMCID: PMC3916430

PRC1 and PRC2 Are Not Required for Targeting of H2A.Z to Developmental Genes in Embryonic Stem Cells
Robert S. Illingworth, Catherine H. Botting, Graeme R. Grimes, Wendy A. Bickmore, Ragnhild Eskeland
PLoS One. 2012; 7(4): e34848.  Published online 2012 Apr 9. doi: 10.1371/journal.pone.0034848
PMCID: PMC3322156

HP1 Binding to Chromatin Methylated at H3K9 Is Enhanced by Auxiliary Factors
Ragnhild Eskeland, Anton Eberharter, Axel Imhof
Mol Cell Biol. 2007 Jan; 27(2): 453–465.  Published online 2006 Nov 13. doi: 10.1128/MCB.01576-06
PMCID: PMC1800810