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DNase I (EC 3.1.21.1)

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€533.11*

Weight
Product number: M3028.0500
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Product information "DNase I (EC 3.1.21.1)"

DNase I is an endonuclease isolated from bovine pancreas that digests double- and single-stranded DNA into oligo- and mono-nucleotides.

DNAse I is used, among other things, to avoid unwanted cell clumping during tissue disaggregation, which makes dispersion of individual cells difficult. Since tissue disaggregation and subsequent isolation of single cells is always accompanied by rupture and lysis of some cells, DNA is released from these cells into the surrounding medium. The released DNA in the medium is thought to be responsible for undesirable cell clumping during tissue dissociation procedures. This clumping effect can be avoided by adding deoxyribonuclease (DNase) to the dissociation medium and has been described for a variety of different cell and tissue types.

DNase I is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RT-PCR. Since no RNA purification procedure removes 100% of the DNA, RNA samples should be digested with DNase I before RT-PCR. A simple 15 minute digestion at room temperature removes the contaminating DNA. DNase I is inactivated by adding the stop solution and heating. Heating also denatures the RNA, so the RNA can be used directly for reverse transcription.

This DNAse I is not RNase-free and has to be chemically treated if used for RNA isolation procedures.

Specifications:
Activity: >3000 units/mg
Appearance: salt-free, freeze-dried powder, chromatographically prepared
MW: appr. 31 kDa


Applikation / Application:
removes DNA for RNA purification procedures. removes DNA in single cell isolation procedures

Einheiten / Units:
Quelle / Source:
bovine pancreas


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

EC-Nr: 232-667-0
CAS-Nr: 9003-98-9
eclass-Nr: 32-16-04-10
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Certificate
Product description
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed


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