Proteinase K Powder, usable for PCR
Advantages at a glance
- Broad-spectrum serin protease with various applications (preparation of chromosomal DNA for PFGE, degradation of nucleases, isolation of genomic DNA)
- Very high purity
- High activity
- Active at denaturing conditions
- Available as powder or solution with different ranges of activity
| Quantity | Unit price |
|---|---|
| To 5 |
€170.50*
€198.50*
(14.11% saved)
|
| To 10 |
€144.93*
€198.50*
(26.99% saved)
|
| From 11 |
€127.88*
€198.50*
(35.58% saved)
|
Ready to ship today,
Delivery time 1-2 workdays
Shipment: not cooled. Store at -20°C. For laboratory usage only!
Overview:
Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.
Product Details:
- ready-to-use
- Concentration: 20 mg/mL
- active over a wide range of reaction conditions
- An elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several times
Quality:
- PCR grade, ensuring high purity and no interference with PCR reactions.
Applications:
- Suitable for DNA and RNA extraction
- Removal of protein contaminants
- Preparation of samples for various molecular biology techniques.
Benefits:
- High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications.
- Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.
- Convenience: Ready-to-use solution at an optimal concentration for ease of use.
Notes:
- The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL.
- The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M urea
- Ca2+ protects Proteinase K against autolysis and increases the thermal stability
- Stable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0
- Activity optimum: 50°C to 55°C
- Rapid denaturation of enzyme occurs at temperatures above 65°C
Features:
- No detected exonuclease, endonuclease or RNase activity
- Specific activity: >40 units/mg protein
- Activity: ≥30 units/mg lyophilizate
- Solubility: ≥20 mg/mL
- DNA content ≤10 pg/mg
- Shipping conditions: Shipment on wet ice
Proteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.
Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.
Specifications:
Appearance: white lyophilisate
Activity: >30 AnsonU/mg protein (>30 units/mg)
DNAses and RNAses: not detectable
Solubility: easily soluble in water
pH (1% H2O, 20°C): 6.2 - 6.8
MW = ca. 27,000 g/mol.
Sicherheits Hinweise / Safety
H-Sätze: H315, H319 ,H334, H335GHS-Symbole: GHS07, GHS08
Klassifizierungen / Classification
EC-Nr: 254-457-8CAS-Nr: 39450-01-6
eclass-Nr: 32-16-04-10
Documents:
Safety Data SheetCertificate
Product description
General Data 1
Source: NCBI PubMed
Decreased Enhancer-Promoter Proximity Accompanying Enhancer Activation
Nezha S. Benabdallah, Iain Williamson, Robert S. Illingworth, Lauren Kane, Shelagh Boyle, Dipta Sengupta, Graeme R. Grimes, Pierre Therizols, Wendy A. Bickmore
Mol Cell. 2019 Nov 7; 76(3): 473–484.e7. doi: 10.1016/j.molcel.2019.07.038
PMCID: PMC6838673
Glucocorticoid Receptor Binding Induces Rapid and Prolonged Large-Scale Chromatin Decompaction at Multiple Target Loci
Alasdair W. Jubb, Shelagh Boyle, David A. Hume, Wendy A. Bickmore
Cell Rep. 2017 Dec 12; 21(11): 3022–3031. Published online 2017 Dec 12. doi: 10.1016/j.celrep.2017.11.053
PMCID: PMC5745231
Enhancer turnover is associated with a divergent transcriptional response to glucocorticoid in mouse and human macrophages
Alasdair W Jubb, Robert S Young, David A Hume, Wendy A Bickmore
J Immunol. Author manuscript; available in PMC 2016 Jul 15.Published in final edited form as: J Immunol. 2016 Jan 15; 196(2): 813–822. Published online 2015 Dec 9. doi: 10.4049/jimmunol.1502009
PMCID: PMC4707550
Active promoters give rise to false positive ‘Phantom Peaks’ in ChIP-seq experiments
Dhawal Jain, Sandro Baldi, Angelika Zabel, Tobias Straub, Peter B. Becker
Nucleic Acids Res. 2015 Aug 18; 43(14): 6959–6968. Published online 2015 Jun 27. doi: 10.1093/nar/gkv637
PMCID: PMC4538825
ISWI Remodelling of Physiological Chromatin Fibres Acetylated at Lysine 16 of Histone H4
Henrike Klinker, Felix Mueller-Planitz, Renliang Yang, Ignasi Forné, Chuan-Fa Liu, Lars Nordenskiöld, Peter B. Becker
PLoS One. 2014; 9(2): e88411. Published online 2014 Feb 6. doi: 10.1371/journal.pone.0088411
PMCID: PMC3916430
PRC1 and PRC2 Are Not Required for Targeting of H2A.Z to Developmental Genes in Embryonic Stem Cells
Robert S. Illingworth, Catherine H. Botting, Graeme R. Grimes, Wendy A. Bickmore, Ragnhild Eskeland
PLoS One. 2012; 7(4): e34848. Published online 2012 Apr 9. doi: 10.1371/journal.pone.0034848
PMCID: PMC3322156
HP1 Binding to Chromatin Methylated at H3K9 Is Enhanced by Auxiliary Factors
Ragnhild Eskeland, Anton Eberharter, Axel Imhof
Mol Cell Biol. 2007 Jan; 27(2): 453–465. Published online 2006 Nov 13. doi: 10.1128/MCB.01576-06
PMCID: PMC1800810
Application tipps
dissolve Proteinase K (20mg/mL) in water or in 50mM Tris/HCl (pH8.0), 1.5mM Ca-Acetate.
Following some application examples based on 20mg/mL concentrations of Proteinase K dissolved in pure water or 50mM Tris, pH8.0.
Standard reaction buffer:
Cell lysis: 50 mM Tris-HCl (pH 7.5); 5 mM CaCl2; 0.5 % SDS.
DNA purification: 100 mM Tris-HCl (pH 8.0); 50 mM EDTA, 500 mM NaCl.
Some Application Protocols:
• Isolation of genomic DNA from mammal cells
(3h, 50°C) 10mM Tris/HCl (pH8.0), 100mM EDTA (pH8.0), 50mM NaCl, 0.5%SDS, 20µg/mL DNase free RNase, 100µg/mL proteinase K.
• Isolation of genomic DNA from mice tails
(overnight, 55°C) 20mM Tris/HCl (pH8.0), 5mM EDTA (pH8.0), 400mM NaCl, 1% SDS, 400µg/mL proteinase K.
• Rapid isolation of genomic DNA as a PCR-template from cell cultures
(1h, 37 °C) 67mM Tris/HCl (pH8.8), 16.6mM ammonium sulfate, 5mM β-mercaptoethanol, 6.7mM MgCl2, 6.7µM EDTA (pH8.0), 1.7µM SDS, 50µg/mL proteinase K.
• Preparation of DNA for PFGE, lysis in agarose block
(approx. 20h, 50°C). 100mM EDTA (pH8.0), 1 mM Tris/HCl (pH7.6), 20mM NaCl, 1% sarcosyl, 100µg/mL proteinase K.
• Purification of mRNA prior to cDNA synthesis (leads often to an increase in cDNA yield)
(1-2h, 37°C) 100mM Tris/HCl (pH7.5), 10mM EDTA (pH8.0), 50mM NaCl, 0.1% SDS, 5µg/mL proteinase K.
• Purification of PCR-reactions prior to cloning
(overnight, 55°C) PCR-reaction + 10mM Tris/HCl (pH8.0), 5mM EDTA (pH8.0), 400mM NaCl, 1% SDS, 400µg/mL proteinase K.
• Purification of plasmid-DNA prior to in vitro transcription
(1h, 37°C) Plasmid-DNA + 21mM Tris/HCl (pH8.0), 5mM EDTA (pH8.0), 50mM NaCl, 0.5% SDS, 100µg/mL proteinase K.
• RNase inactivation in ribonuclease-protection-assays
(30 min, 37°C) 30µL RNA/RNA-hybridisation mix + 300µL RNase digestion mix + 0.6% SDS, 300µg/mL proteinase K.
• Inactivation of DNase in transcription-run-on-assays
(30 min, 42°C) Suspension of nuclei in 36mM Tris/HCl (pH7.4), 17mM MgCl2, 0.7mM CaCl2, 170mM NaCl, 13mM EDTA (pH8.0), 0.5% SDS with DNase, 100µg/mL proteinase K.
• Denaturation of alkaline phosphatase when cipping vector DNA
(30 min, 56°C) 1 x dephosphorylation buffer + 0.5% SDS, 5mM EDTA (pH8.0), 100µg/mL proteinase K.
