Taq DNA Polymerase S (high specificity)

Taq Polymerase from Genaxxon


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High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase,... more
Product information "Taq DNA Polymerase S (high specificity)"

High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15mM MgCl2.

Free Taq DNA Polymerase test sample available!No shipping costs within Germany.

With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

More products around "Taq DNA Polymerase S (high specificity)"
Product Specifications Concentration: 5 units/µL Substrate analogs: dNTP, ddNTP, fluorescent... more
 

Technical Data:

Product Specifications
Concentration: 5 units/µL
Substrate analogs: dNTP, ddNTP, fluorescent dNTP/ddNTP
Extension rate: 2-4 kb/min. at 72°C
Half-life: 20min. at 95°C, 60min. at 94°C
5’-3’ exonuclease activity: Yes
Extra addition of A: Yes
3’-5’ exonuclease activity: No
Nuclease contamination: No
Protease contamination: No
RNase contamination: No
Self-priming activity: No

application:

DNA polymerase to be used for standard PCR applications.

Unit Definition:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Source

Thermus aquaticus strain

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Taq DNA Polymerase S (high specificity)

 
 
 
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

For every template/primer pair, the optimal reaction conditions have to be evaluated empirically, changing the primer/template
ratio, the ionic strength (with MgSO4) and the cycle parameters (time and temperatures).

DNA Template

Amplification of templates with high GC content, strong secondary structure, low concentrations, or which produce products greater than 5 kb, may require adaptation of the following parameters:

  • Use high quality, purified DNA templates.
  • Approximately 10E4 copies of target DNA are required to detect product in 25-30 PCR cycles.
  • Use 1pg–1ng of plasmid or viral templates.
  • Use 1ng–1µg of genomic templates.
  • Higher DNA concentrations decrease amplicon specificity (i.e., extra bands are more likely), particularly when a large number of cycles are employed.
  • Use the higher DNA concentrations when fewer cycles are desired (e.g. to increase fidelity).

Primers

  • Generally 20-30 nucleotides in length.
  • Ideal GC content is 40-60%.
  • Space GC residues evenly within the primer.
  • Primer pairs should have Tms within 5°C of each other.
  • Avoid secondary structure (i.e., hairpins) within each primer and potential dimerization between the primers present.
  • When engineering sites into the end of primers, 4-6 extra bases should be added 5´ to the site.
  • Final concentration should be 0.05-1 µM, typically 0.1-0.5 µM of each primer.
  • Higher concentrations may increase secondary priming and create spurious amplification products.

Magnesium Concentration

  • 1.5-2.0 mM is optimal for Taq DNA Polymerase, but the ideal concentration depends on template, buffer, DNA and dNTPs (each has the potential to chelate magnesium).
  • If [Mg2+] is too low, no PCR product will be seen.
  • If [Mg2+] is too high, undesired PCR products may be seen.

Deoxyribonucleotide triphosphates (dNTPs) >

  • Typical concentration is 200 µM of each dNTP.
  • 50-100 µM enhances fidelity of polymerization, but reduces yields.
  • Higher concentrations increase yields particularly in long PCR, but can reduce fidelity.

DNA Polymerase

  • The choice of the correct polymerase depends among other things on the purpose as well as on the template used (standard PCR: Taq DNA Polymerase S > with high accuracy, Taq Polymerase E > with high yield; master mixes: Standard PCR master mix > or RedMasterMix > with red dye).
  • For multiplex PCR, there exist special multiplex master mixes >.
  • Hot start applications are recommended to increase specificity or if you use difficult templates. For this purpose there exist special Hot Start Polymerases >.
  • For PCRs for the purpose of cloning or other procedures requiring a low error rate, there are thermostable high fidelity proofing polymerases such as Pfunds >, ExactRun >, ReproFast >, or ReproHot (KOD) Proofreading Polymerase >. These enzymes make far fewer errors during amplification and increase the chances of an amplicon without mistakes.
  •  For genotyping and other applications where a high discrimination rate is required, there is a new highly selective DNA polymerase, SNP Pol DNA Polymerase >. It specifically distinguishes mismatched primer-template complexes and ony produces specific amplicons in case of perfectly matched primer pairs.
  • For the real-time quantitative PCR > there exist highly specialized qPCR master mixes. Hereby, the choice of the correct master mix depends on the qPCR device you are using. It is important to determine whether and how much ROX should be contained in the qPCR master mix and whether it should be a qPCR green master mix with green fluorescent dye > or a qPCR probe  master mix > without a fluorescent dye. A new lyophilized qPCR probe master mix in beads format, which is stable at room temperature, is available from Genaxxon (LyoBalls >).
  • The amount of DNA polymerase used in the PCR reaction can significantly influence the PCR result (use 1.25-1.5 units Taq Polymerase > for a 50μL volume).

General Guidelines

Annealing Temperature and Duration

  • Match the Tms within 5°C of each other.
  • Typical annealing temperatures are 5°C below the lowest primer's Tm and often fall in the range of 50-60°C.
  • Test higher annealing temperatures if spurious amplification products are observed.
  • Typical annealing times are 15-30 seconds.

Extension Time

  • Extensions are normally performed at 68°C.
  • In general, use extension times of one minute per 1000 base (1 kb) pairs (e.g. 3 minutes for a 3 kb product). 
  • For products less than 1 kb, use 45-60 seconds.
  • Products greater than 3 kb, or reactions using more than 30 cycles, may require longer extensions.

The amplification of templates with high GC content, strong secondary structures, low concentrations or amplicons more than 5 kb often require optimization of the PCR conditions. Typically, 15-30 seconds of denaturation should be performed at 95 ° C during PCR.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis

Julian Lange, Eugenia Münch, Jan Müller, Tobias Busche, Jörn Kalinowski, Ralf Takors, Bastian Blombach

Genes (Basel) 2018 Jun; 9(6): 297. Published online 2018 Jun 13. doi: 10.3390/genes9060297

PMCID: PMC6027265

 

Identification, differentiation and antibiotic susceptibility of Gallibacterium isolates from diseased poultry

Hosny El-Adawy, Herbert Bocklisch, Heinrich Neubauer, Hafez Mohamed Hafez, Helmut Hotzel

Ir Vet J. 2018; 71: 5. Published online 2018 Feb 5. doi: 10.1186/s13620-018-0116-2

PMCID: PMC5799919

 

An application of competitive reporter monitored amplification (CMA) for rapid detection of single nucleotide polymorphisms (SNPs)

Juliane Havlicek, Eric Rivera-Milla, Peter Slickers, Sönke Andres, Silke Feuerriegel, Stefan Niemann, Matthias Merker, Ines Labugger

PLoS One. 2017; 12(8): e0183561. Published online 2017 Aug 29. doi: 10.1371/journal.pone.0183561

PMCID: PMC5574540

 

The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome

Coral González-Prieto, Richard Gabriel, Christoph Dehio, Manfred Schmidt, Matxalen Llosa

Appl Environ Microbiol. 2017 Jun 15; 83(12): e00207-17. Prepublished online 2017 Apr 14. Published online 2017 May 31. doi: 10.1128/AEM.00207-17

PMCID: PMC5452801

 

Genetic alterations in seborrheic keratoses

Barbara Heidenreich, Evygenia Denisova, Sivaramakrishna Rachakonda, Onofre Sanmartin, Timo Dereani, Ismail Hosen, Eduardo Nagore, Rajiv Kumar

Oncotarget. 2017 May 30; 8(22): 36639–36649. Published online 2017 Mar 30. doi: 10.18632/oncotarget.16698

PMCID: PMC5482683

 

Post-translational Serine/Threonine Phosphorylation and Lysine Acetylation: A Novel Regulatory Aspect of the Global Nitrogen Response Regulator GlnR in S. coelicolor M145

Rafat Amin, Mirita Franz-Wachtel, Yvonne Tiffert, Martin Heberer, Mohamed Meky, Yousra Ahmed, Arne Matthews, Sergii Krysenko, Marco Jakobi, Markus Hinder, Jane Moore, Nicole Okoniewski, Boris Maček, Wolfgang Wohlleben, Agnieszka Bera

Front Mol Biosci. 2016; 3: 38.  Published online 2016 Aug 9. doi: 10.3389/fmolb.2016.00038

PMCID: PMC4977719

 

Quantification of Slackia and Eggerthella spp. in Human Feces and Adhesion of Representatives Strains to Caco-2 Cells

Gyu-Sung Cho, Felix Ritzmann, Marie Eckstein, Melanie Huch, Karlis Briviba, Diana Behsnilian, Horst Neve, Charles M. A. P. Franz

Front Microbiol. 2016; 7: 658.  Published online 2016 May 9. doi: 10.3389/fmicb.2016.00658

PMCID: PMC4860493

 

Transcriptional Regulation of the β-Type Carbonic Anhydrase Gene bca by RamA in Corynebacterium glutamicum

Adnan Shah, Bernhard J. Eikmanns

PLoS One. 2016; 11(4): e0154382.  Published online 2016 Apr 27. doi: 10.1371/journal.pone.0154382

PMCID: PMC4847777

 

Mapping of deletion breakpoints at the CDKN2A locus in melanoma: detection of MTAP-ANRIL fusion transcripts

Huaping Xie, P. Sivaramakrishna Rachakonda, Barbara Heidenreich, Eduardo Nagore, Antje Sucker, Kari Hemminki, Dirk Schadendorf, Rajiv Kumar

Oncotarget. 2016 Mar 29; 7(13): 16490–16504.  Published online 2016 Feb 19. doi: 10.18632/oncotarget.7503

PMCID: PMC4941330

 

DNase-Sensitive and -Resistant Modes of Biofilm Formation by Listeria monocytogenes

Marion Zetzmann, Mira Okshevsky, Jasmin Endres, Anne Sedlag, Nelly Caccia, Marc Auchter, Mark S. Waidmann, Mickaël Desvaux, Rikke L. Meyer, Christian U. Riedel

Front Microbiol. 2015; 6: 1428.  Published online 2015 Dec 22. doi: 10.3389/fmicb.2015.01428

PMCID: PMC4686886

 

Skin-Derived C-Terminal Filaggrin-2 Fragments Are Pseudomonas aeruginosa-Directed Antimicrobials Targeting Bacterial Replication

Britta Hansmann, Jens-Michael Schröder, Ulrich Gerstel

PLoS Pathog. 2015 Sep; 11(9): e1005159.  Published online 2015 Sep 15. doi: 10.1371/journal.ppat.1005159

PMCID: PMC4570713

 

Tight Junction Protein 1a regulates pigment cell organisation during zebrafish colour patterning

Andrey Fadeev, Jana Krauss, Hans Georg Frohnhöfer, Uwe Irion, Christiane Nüsslein-Volhard

eLife. 2015; 4: e06545.  Published online 2015 Apr 27. doi: 10.7554/eLife.06545Includes additional comments & authors

PMCID: PMC4446668

 

Down-Regulation of ZmEXPB6 (Zea mays β-Expansin 6) Protein Is Correlated with Salt-mediated Growth Reduction in the Leaves of Z. mays L.

Christoph-Martin Geilfus, Dietrich Ober, Lutz A. Eichacker, Karl Hermann Mühling, Christian Zörb

J Biol Chem. 2015 May 1; 290(18): 11235–11245.  Published online 2015 Mar 6. doi: 10.1074/jbc.M114.619718

PMCID: PMC4416831

 

Phylogeny and Differentiation of Reptilian and Amphibian Ranaviruses Detected in Europe

Anke C. Stöhr, Alberto López-Bueno, Silvia Blahak, Maria F. Caeiro, Gonçalo M. Rosa, António Pedro Alves de Matos, An Martel, Alí Alejo, Rachel E. Marschang

PLoS One. 2015; 10(2): e0118633.  Published online 2015 Feb 23. doi: 10.1371/journal.pone.0118633

PMCID: PMC4338083

 

The Staphylococcus aureus NuoL-Like Protein MpsA Contributes to the Generation of Membrane Potential

Sonja Mayer, Wojtek Steffen, Julia Steuber, Friedrich Götz

J Bacteriol. 2015 Mar; 197(5): 794–806.  Prepublished online 2014 Dec 1. Published online 2015 Feb 4. doi: 10.1128/JB.02127-14

PMCID: PMC4325100

 

Transcriptional landscape and essential genes of Neisseria gonorrhoeae

Christian W. Remmele, Yibo Xian, Marco Albrecht, Michaela Faulstich, Martin Fraunholz, Elisabeth Heinrichs, Marcus T. Dittrich, Tobias Müller, Richard Reinhardt, Thomas Rudel

Nucleic Acids Res. 2014 Sep 15; 42(16): 10579–10595.  Published online 2014 Aug 20. doi: 10.1093/nar/gku762

PMCID: PMC4176332

 

 

Molecular evidence for convergent evolution and allopolyploid speciation within the Physcomitrium-Physcomitrella species complex

Anna K Beike, Mark von Stackelberg, Mareike Schallenberg-Rüdinger, Sebastian T Hanke, Marie Follo, Dietmar Quandt, Stuart F McDaniel, Ralf Reski, Benito C Tan, Stefan A Rensing

BMC Evol Biol. 2014; 14: 158.  Published online 2014 Jul 11. doi: 10.1186/1471-2148-14-158

PMCID: PMC4227049

 

Improvement of L-phenylalanine production from glycerol by recombinant Escherichia coli strains: The role of extra copies of glpK, glpX, and tktA genes

Katrin Gottlieb, Christoph Albermann, Georg A Sprenger

Microb Cell Fact. 2014; 13: 96.  Published online 2014 Jul 11. doi: 10.1186/s12934-014-0096-1

PMCID: PMC4227036

 

Molecular Typing of MRSA and of Clinical Staphylococcus aureus Isolates from Iaşi, Romania

Stefan Monecke, Elke Müller, Olivia Simona Dorneanu, Teodora Vremeră, Ralf Ehricht

PLoS One. 2014; 9(5): e97833.  Published online 2014 May 20. doi: 10.1371/journal.pone.0097833

PMCID: PMC4028265

 

Bioelectric Signaling Regulates Size in Zebrafish Fins

Simon Perathoner, Jacob M. Daane, Ulrike Henrion, Guiscard Seebohm, Charles W. Higdon, Stephen L. Johnson, Christiane Nüsslein-Volhard, Matthew P. Harris

PLoS Genet. 2014 Jan; 10(1): e1004080.  Published online 2014 Jan 16. doi: 10.1371/journal.pgen.1004080

PMCID: PMC3894163

 

The PHOTOSYNTHESIS AFFECTED MUTANT68–LIKE Protein Evolved from a PSII Assembly Factor to Mediate Assembly of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis

Ute Armbruster, Thilo Rühle, Renate Kreller, Christoph Strotbek, Jessica Zühlke, Luca Tadini, Thomas Blunder, Alexander P. Hertle, Yafei Qi, Birgit Rengstl, Jörg Nickelsen, Wolfgang Frank, Dario Leister

Plant Cell. 2013 Oct; 25(10): 3926–3943.  Published online 2013 Oct 4. doi: 10.1105/tpc.113.114785

PMCID: PMC3877787

 

Variants at the 9p21 locus and melanoma risk

Livia Maccioni, Panduranga Sivaramakrishna Rachakonda, Justo Lorenzo Bermejo, Dolores Planelles, Celia Requena, Kari Hemminki, Eduardo Nagore, Rajiv Kumar

BMC Cancer. 2013; 13: 325.  Published online 2013 Jul 2. doi: 10.1186/1471-2407-13-325

PMCID: PMC3702420

 

Phylogenetic and Molecular Analysis of Food-Borne Shiga Toxin-Producing Escherichia coli

Elisabeth Hauser, Alexander Mellmann, Torsten Semmler, Helen Stoeber, Lothar H. Wieler, Helge Karch, Nikole Kuebler, Angelika Fruth, Dag Harmsen, Thomas Weniger, Erhard Tietze, Herbert Schmidt

Appl Environ Microbiol. 2013 Apr; 79(8): 2731–2740.  doi: 10.1128/AEM.03552-12

PMCID: PMC3623172

 

German Francisella tularensis isolates from European brown hares (Lepus europaeus) reveal genetic and phenotypic diversity

Wolfgang Müller, Helmut Hotzel, Peter Otto, Axel Karger, Barbara Bettin, Herbert Bocklisch, Silke Braune, Ulrich Eskens, Stefan Hörmansdorfer, Regina Konrad, Anne Nesseler, Martin Peters, Martin Runge, Gernot Schmoock, Bernd-Andreas Schwarz, Reinhard Sting, Kerstin Myrtennäs, Edvin Karlsson, Mats Forsman, Herbert Tomaso

BMC Microbiol. 2013; 13: 61.  Published online 2013 Mar 21. doi: 10.1186/1471-2180-13-61

PMCID: PMC3663675

 

Increased detection rates of EGFR and KRAS mutations in NSCLC specimens with low tumour cell content by 454 deep sequencing

Evgeny A. Moskalev, Robert Stöhr, Ralf Rieker, Simone Hebele, Florian Fuchs, Horia Sirbu, Sergey E. Mastitsky, Carsten Boltze, Helmut König, Abbas Agaimy, Arndt Hartmann, Florian Haller

Virchows Arch. 2013 Apr; 462(4): 409–419.  Published online 2013 Mar 7. doi: 10.1007/s00428-013-1376-6

PMCID: PMC3624006

 

An efficient method for genome-wide polyadenylation site mapping and RNA quantification

Stefan Wilkening, Vicent Pelechano, Aino I. Järvelin, Manu M. Tekkedil, Simon Anders, Vladimir Benes, Lars M. Steinmetz

Nucleic Acids Res. 2013 Mar; 41(5): e65.  Published online 2013 Jan 7. doi: 10.1093/nar/gks1249Correction in: Nucleic Acids Res. 2013 Jul; 41(12): 6370.

PMCID: PMC3597643

 

Characterization of a mazEF Toxin-Antitoxin Homologue from Staphylococcus equorum

Christopher F. Schuster, Jung-Ho Park, Marcel Prax, Alexander Herbig, Kay Nieselt, Ralf Rosenstein, Masayori Inouye, Ralph Bertram

J Bacteriol. 2013 Jan; 195(1): 115–125.  doi: 10.1128/JB.00400-12

PMCID: PMC3536171

 

Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus

Anna C. Shore, Emily C. Deasy, Peter Slickers, Grainne Brennan, Brian O'Connell, Stefan Monecke, Ralf Ehricht, David C. Coleman

Antimicrob Agents Chemother. 2011 Aug; 55(8): 3765–3773.  doi: 10.1128/AAC.00187-11

PMCID: PMC3147645

 

Quantitative Trait Loci Involved in Sex Determination and Body Growth in the Gilthead Sea Bream (Sparus aurata L.) through Targeted Genome Scan

Dimitrios Loukovitis, Elena Sarropoulou, Costas S. Tsigenopoulos, Costas Batargias, Antonios Magoulas, Apostolos P. Apostolidis, Dimitrios Chatziplis, Georgios Kotoulas

PLoS One. 2011; 6(1): e16599.  Published online 2011 Jan 31. doi: 10.1371/journal.pone.0016599

PMCID: PMC3031595

 

The Apparent Malate Synthase Activity of Rhodobacter sphaeroides Is Due to Two Paralogous Enzymes, (3S)-Malyl-Coenzyme A (CoA)/β-Methylmalyl-CoA Lyase and (3S)- Malyl-CoA Thioesterase

Tobias J. Erb, Lena Frerichs-Revermann, Georg Fuchs, Birgit E. Alber

J Bacteriol. 2010 Mar; 192(5): 1249–1258.  Published online 2010 Jan 4. doi: 10.1128/JB.01267-09

PMCID: PMC2820834

 

Highlights of the Didymellaceae: A polyphasic approach to characterise Phoma and related pleosporalean genera

M.M. Aveskamp, J. de Gruyter, J.H.C. Woudenberg, G.J.M. Verkley, P.W. Crous

Stud Mycol. 2010; 65: 1–60.  doi: 10.3114/sim.2010.65.01

PMCID: PMC2836210

 

Molecular Analysis of Virulence Profiles and Shiga Toxin Genes in Food-Borne Shiga Toxin-Producing Escherichia coli

T. Slanec, A. Fruth, K. Creuzburg, H. Schmidt

Appl Environ Microbiol. 2009 Oct; 75(19): 6187–6197.  Published online 2009 Aug 14. doi: 10.1128/AEM.00874-09

PMCID: PMC2753087

 

A trans-splicing group I intron and tRNA-hyperediting in the mitochondrial genome of the lycophyte Isoetes engelmannii

Felix Grewe, Prisca Viehoever, Bernd Weisshaar, Volker Knoop

Nucleic Acids Res. 2009 Aug; 37(15): 5093–5104.  Published online 2009 Jun 23. doi: 10.1093/nar/gkp532

PMCID: PMC2731911

 

A Microsatellite-Based Genetic Linkage Map of the Cichlid Fish, Astatotilapia burtoni (Teleostei): A Comparison of Genomic Architectures Among Rapidly Speciating Cichlids

Matthias Sanetra, Frederico Henning, Shoji Fukamachi, Axel Meyer

Genetics. 2009 May; 182(1): 387–397.  doi: 10.1534/genetics.108.089367

PMCID: PMC2674835

 

Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype

J.H.C. Woudenberg, M.M. Aveskamp, J. de Gruyter, A.G. Spiers, P.W. Crous

Persoonia. 2009 Jun; 22: 56–62.  Published online 2009 Mar 3. doi: 10.3767/003158509X427808

PMCID: PMC2789541

 

Sexual Reproduction as the Cause of Heat Resistance in the Food Spoilage Fungus Byssochlamys spectabilis (Anamorph Paecilomyces variotii)

Jos Houbraken, János Varga, Emilia Rico-Munoz, Shawn Johnson, Robert A. Samson

Appl Environ Microbiol. 2008 Mar; 74(5): 1613–1619.  Published online 2008 Jan 11. doi: 10.1128/AEM.01761-07

PMCID: PMC2258620

 

Molecular Characterization and Distribution of Genes Encoding Members of the Type III Effector NleA Family among Pathogenic Escherichia coli Strains

Kristina Creuzburg, Herbert Schmidt

J Clin Microbiol. 2007 Aug; 45(8): 2498–2507.  Published online 2007 Jun 6. doi: 10.1128/JCM.00038-07

PMCID: PMC1951211

 

Nuclear Factor I X Deficiency Causes Brain Malformation and Severe Skeletal Defects

Katrin Driller, Axel Pagenstecher, Markus Uhl, Heymut Omran, Ansgar Berlis, Albert Gründer, Albrecht E. Sippel

Mol Cell Biol. 2007 May; 27(10): 3855–3867.  Published online 2007 Mar 12. doi: 10.1128/MCB.02293-06

PMCID: PMC1899988

 

Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution

Kristian M. Müller, Sabine C. Stebel, Susanne Knall, Gregor Zipf, Hubert S. Bernauer, Katja M. Arndt

Nucleic Acids Res. 2005; 33(13): e117.  Published online 2005 Aug 1. doi: 10.1093/nar/gni116

PMCID: PMC1182171

 
 
 
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