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ReproHot (KOD polymerase) is a high fidelity DNA polymerase for accurate PCR amplification of GC-rich templates used for cloning and cDNA amplification. This enzyme is a mixture of Taq DNA polymerase plus a proofreading polymerase and a monoclonal antibody, which blocks polymerase activity prior to heating. The complex formed between the polymerase and the antibody prevents primer-dimer and other artifacts resulting from unspecific primer binding. The antibody is inactivated while heating up above 75°C, requiring no prolonged first heating step prior to cycling. The combined hotstart and proofreading feature leads to highly specific and correct amplicons. ReproHot can be used as a cheap alternative to KOD-Polymerase from. e.g. Merck Millipore or other sources.
Other proofreading enzymes (Phusion, Pfu, Pwo, etc.).
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.
Anitbody based Hotstart DNA polymerase with proof-reading activity.