Order number: A223102Shipping: shipped on wet ice, store at -20°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
About TEMPase Hot Start DNA Polymerase
TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase allows for detection of low copy number targets and for multiplex PCR in a single reaction tube. TEMPase Hot Start DNA Polymerase is available together with different buffers. This one (A2251xx) is supplied with 10X ammonium sulfate buffer plus 10X combination buffer plus separate 25mM MgCl2.
TEMPase Hot Start DNA Polymerase is also available together with 10X ammonium sulfate buffer and separate 25mM MgCl2 (A2211xx).
- Reaction set up at room temperature
- Increased sensitivity
- Increased specificity
- Increased product yield
TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase, which is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended.
Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a heat activation step, releasing the active TEMPase Hot Start DNA Polymerase into the reaction.
Please check also our SuperHot Hot Start Mastermix > with or without tracking dye.
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.
Enyzme is completely inactive prior to heating at 95°C for 15 min.!
application:for multiplex PCR for Real-time PCR for PCR of low amount of DNA (very low copy number)
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on TEMPase Hot Start DNA Polymerase. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.