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2',3'-Dideoxythymidine-5'-O-triphosphate (ddTTP) - 10mM solution

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  • High-quality products
  • Customized solutions
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  • Fast service
  • Competent technical support +49(0)731-3608-123

€824.16*

amount of substance
Product number: M3459.0025
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

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Product information "2',3'-Dideoxythymidine-5'-O-triphosphate (ddTTP) - 10mM solution"

3'-Deoxythymidine-5'-O-triphosphate (2',3'-Dideoxythymidine-5'-O-triphosphate) (dTTP/ddTTP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. Note: Since “thymidine“ already describes a 2'- deoxy nucleosides, the term “2'-deoxythymidine“ is redundant.

3'-Deoxythymidine-5'-O-triphosphate (dTTP/ddTTP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination while PCR and is widely useful in antiviral research and in a variety of biotechnology applications. dTTP/ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases.

Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.

Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977).

dTTP/ddTTP is an analogue of thymidine triphosphate (TTP) where both ribose hydroxy groups have been removed and replaced by hydrogen.

Amount and packaging:
The product is delivered as 500µL of a10mM solution, pH7.6 (500µL correspond to 5µmol (appr. 2.3mg)).

Specifications:
Purity: min. 95% (HPLC)
10mM solution, pH7.6 (non-sterile, not tested for endotoxins)
λmax = 267 nm; ε = 9600 λ x mmol-1 x cm-1
C10H17N2O13P3
MW = 466.2 g/mol (free acid)


Applikation / Application:
Einheiten / Units:
Quelle / Source:
synthetic


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

CAS-Nr: 128524-26-5
eclass-Nr: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Safety Data Sheet
Product description
General Data 1
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed