dUTP solution 100mM

Order number: M3022.0020
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Deoxyuridine 5'-triphosphate, tetrasodium salt (dUTP) of purity >99% (HPLC). dUTP may be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. Product has been tested for PCR products of length up to 8kb (Taq DNA polymerase) and 0.8kb (Pfu DNA polymerase). The substitution of dTTP for dUTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme Uracil-DNA-Glycosylase, UDG >, can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives.
- Purity: more than 99% pure (HPLC) - free of human or E.coli DNA - very stable - stable for years if stored at -20°C - stable also after several freeze-thaw cycles
Technical Data:
Specifications:
Purity: min. 99% (HPLC)
UV-spectrum: confirms
UV-ratios: 250/260nm: 0.74 +/-0.03; 280/260nm: 0.38 +/-0.02; 290/260nm: 0.04 +/-0.01
ε = 9.8 λ x mmol-1 x cm-1
C9H15N2O14P3 (free acid)
MW = 468 g/mol
1mL corresponds to 100 µmol
Source
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Here you will find information and further literature on dUTP solution 100mM. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.