Biotin-11-dUTP Solution, min. 96% (1mM)

Biotin-11-dUTP (Biotin-11-2'-deoxyuridin-5'-triphosphat - Tetralithiumsalt) is a commonly used component for non-radioactive labelling of DNA. Biotin-11-dUTP can be incorporated into DNA enzymatically by Nick-Translation, random-priming, 3'-end terminal labelling or by PCR. The figure '11' stands for the number of carbon atoms of the linker between dUTP and Biotin. The longer the 'linker-chain' the better the interaction between Biotin and Avidin. The shorter the linker the better the incorporation of dUTP into DNA. '11' has been shown to be a good compromise for most applications. The product Biotin-11-dUTP for non-radioactive labelling of DNA is delivered as 1 mM solution in 10 mM Tris-HCl (pH7.5), 1 mM EDTA.

Biotin-11-dUTP can be enzymatically incorporated into DNA with Taq DNA Polymerase >, phi29 DNA Polymerase, Klenow Fragment, and DNA Polymerase I.

Besides Biotin-11-dUTP Genaxxon offers other modified nucleotides or non modified dNTPs as our PCR dNTP-Mix> with 2 mM > or 10 mM > respective our dNTP-Set 100mM >.

You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001) >, or our proof-reading polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as the ready-to-use RedMastermix (M3029) >, already including dNTPs and a red dye.