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2',3'-Dideoxyadenosine-5'-O-triphosphate (ddATP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire.
2',3'-Dideoxyadenosine-5'-Triphosphate (ddATP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination while PCR. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is widely useful in antiviral research and in a variety of biotechnology applications. ddATP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases.
Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977).
ddATP is an analogue of cyditine triphosphate (ATP) where both ribose hydroxy groups have been removed and replaced by hydrogen.
Purity: min. 95% (HPLC)
λmax = 259 nm; ε = 15000.01 λ x mmol-1 x cm-1
MW = 475.2 g/mol (free acid)
500µL of 10mM sodium salt solution
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 132619-65-9
Dokumente - Protokolle - Downloads
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