2',3'-Dideoxythymidine-5'-O-triphosphate (ddTTP) - 10mM solution

dTTP - ddTTP


Order number: M3459.0005

Shipping: shipped on wet ice, store at -70°C
CAS-Nr: 128524-26-5

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€264.60 *

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3'-Deoxythymidine-5'-O-triphosphate (2',3'-Dideoxythymidine-5'-O-triphosphate) (dTTP/ddTTP)... more
Product information "2',3'-Dideoxythymidine-5'-O-triphosphate (ddTTP) - 10mM solution"

3'-Deoxythymidine-5'-O-triphosphate (2',3'-Dideoxythymidine-5'-O-triphosphate) (dTTP/ddTTP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. Note: Since “thymidine“ already describes a 2'- deoxy nucleosides, the term “2'-deoxythymidine“ is redundant.

3'-Deoxythymidine-5'-O-triphosphate (dTTP/ddTTP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination while PCR and is widely useful in antiviral research and in a variety of biotechnology applications. dTTP/ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases.

Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.

Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977).

dTTP/ddTTP is an analogue of thymidine triphosphate (TTP) where both ribose hydroxy groups have been removed and replaced by hydrogen.

Amount and packaging:
The product is delivered as 500µL of a10mM solution, pH7.6 (500µL correspond to 5µmol (appr. 2.3mg)).

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Our comment on "2',3'-Dideoxythymidine-5'-O-triphosphate (ddTTP) - 10mM solution"
Typical purity is better than 95% (HPLC / UV / 267 nm) at time of quality control and packing. However, actual purity depends on storage and transport conditions. The product is not sterile and has not been tested for endotoxins.
Specifications: Purity: min. 95% (HPLC) 10mM solution, pH7.6 (non-sterile, not tested for... more
 

Technical Data:

Specifications:
Purity: min. 95% (HPLC)
10mM solution, pH7.6 (non-sterile, not tested for endotoxins)
λmax = 267 nm; ε = 9600 λ x mmol-1 x cm-1
C10H17N2O13P3
MW = 466.2 g/mol (free acid)

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

CAS-Nr: 128524-26-5
eclass-Nr: 32-16-05-02
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on 2',3'-Dideoxythymidine-5'-O-triphosphate (ddTTP) - 10mM solution. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
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