dNTP Mix (Na salt) - 10mM

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Quantity	Unit price
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Volume

200 µL

1 mL

Product number: M3016.0200
Ready to ship today,
Delivery time 1-2 workdays

Shipment: on wet ice. Store at -20°C. For laboratory usage only!

Product information "dNTP Mix (Na salt) - 10mM"

Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods.

The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions.

Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP)
Concentration: 10 mM each nucleotide

Please have also a look on our broad range of nucleotides > especially the dNTP mix with 2 mM dNTP mix > our dNTP set > modified nucleotides, e.g. Biotin-11-dUTP >. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001) >, or our proof-reading polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as the ready-to-use RedMastermix (M3029) >, already including dNTPs.

FAQs

dNTPs (deoxynucleoside triphosphates) are the building blocks of DNA. In both PCR and qPCR, the quality, stability, and quantity of dNTPs are crucial. Ensuring their purity is particularly vital when you're dealing with small starting amounts of DNA template.

Genaxxon offers dNTPs in two different forms: either as a set or a premixed solution. The mix conveniently provides all four types—dATP, dCTP, dGTP, and dTTP—in a single tube, each at a specific concentration. These mixes are designed for immediate use, meticulously fine-tuned for PCR and qPCR tasks. On the other hand, the dNTP set offers individual tubes for each dNTP, providing flexibility and control over their usage.

Typically, a standard concentration for each dNTP in a PCR setup is 0.2mM (concentrations from 0.2mM to 0.4mM are usually ideal for your PCR). Lower concentrations, between 0.05mM to 0.1mM, may enhance PCR specificity but could reduce product yield. Conversely, higher concentrations may boost yield but at the expense of specificity. Longer PCR fragments might necessitate higher dNTP concentrations. However, adjusting dNTP concentration requires corresponding adjustments in other PCR components like buffer and Mg2+.

Typically, increasing the amount of dNTPs in your PCR can enhance DNA yield but might compromise specificity. Please note that it’s also necessary adjusting other PCR components such as buffer and Mg2+ if you increase the dNTP concentration.

The PCR assay can be significantly impacted by inhibitors present in dNTP solutions that are not ultra-pure, often stemming from inadequate manufacturing processes. Ideally, dNTPs should be free of impurities such as ribonucleoside triphosphates, additional dNTPs, modified nucleotides, deoxynucleoside di- and monophosphates, heavy transition metals, or other inhibitors.

Low-quality dNTPs may contain modified nucleotides. When using a standard Taq polymerase, point mutations may occur because the enzyme cannot distinguish between different dNTPs. Using a proofreading polymerase can only partially address this issue, as the presence of modified nucleotides can also completely block DNA synthesis.

dNTPs remain stable when stored at -20°C or -70°C in a consistent-temperature freezer for at least 24 months. If stored at room temperature (+15°C to +30°C), they can be kept for up to one week. To maintain their stability, it's best to avoid repeated thawing and freezing cycles. For prolonged storage, it's advisable to use aliquots.

Specifications:
10 mmol/L mix of each dNTP (dATP, dCTP, dGTP, dTTP).
Sodium salt of each dNTP
Purity of each dNTP: min. 99% (HPLC)
No DNases, RNases, proteases detectable. No human or E.coli DNA detectable.

1mL corresponds to 10 µmol of each dNTP.

dATP: λmax 259nm; e 15.4 λ mmol-1 cm-1 (Tris/HCl, pH7.0)
dCTP: λmax 271nm; e 8.9 λ mmol-1 cm-1 (Tris/HCl, pH7.0)
dGTP: λmax 252nm; e 13.7 λ mmol-1 cm-1 (Tris/HCl, pH7.0)
dTTP: λmax 262nm; e 9.6 λ mmol-1 cm-1 (Tris/HCl, pH7.0)

Applikation / Application:

PCR qPCR Multiplex PCR

Einheiten / Units:

Quelle / Source:

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-05-02

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Safety Data Sheet
Certificate
Product description
General Data 1

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

Quelle/Source: NCBI PubMed >

Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces
Christian Weisenstein, Dominik Schaar, Anna Katharina Wigger, Heiko Schäfer-Eberwein, Anja K. Bosserhoff, Peter Haring Bolívar
Biomed Opt Express. 2020 Jan 1; 11(1): 448–460. Published online 2019 Dec 23. doi: 10.1364/BOE.380818
PMCID: PMC6968760

Monitoring the threatened utility of malaria rapid diagnostic tests by novel high-throughput detection of Plasmodium falciparum hrp2 and hrp3 deletions: A cross-sectional, diagnostic accuracy study
Andrea Kreidenweiss, Franziska Trauner, Miriam Rodi, Erik Koehne, Jana Held, Lea Wyndorps, Gédéon Prince Manouana, Matthew McCall, Ayola Akim Adegnika, Albert Lalremruata, Peter G. Kremsner, Rolf Fendel, Thaisa Lucas Sandri
EBioMedicine. 2019 Dec; 50: 14–22. Published online 2019 Nov 21. doi: 10.1016/j.ebiom.2019.10.048
PMCID: PMC6921222

The circadian clock is functional in eosinophils and mast cells
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Immunology. 2013 Dec; 140(4): 465–474. Published online 2013 Oct 28. doi: 10.1111/imm.12157
PMCID: PMC3839650

Low Density Lipoprotein Receptor-related Protein 1 (LRP1) Modulates N-Methyl-d-aspartate (NMDA) Receptor-dependent Intracellular Signaling and NMDA-induced Regulation of Postsynaptic Protein Complexes
Chikako Nakajima, Akos Kulik, Michael Frotscher, Joachim Herz, Michael Schäfer, Hans H. Bock, Petra May
J Biol Chem. 2013 Jul 26; 288(30): 21909–21923. Published online 2013 Jun 11. doi: 10.1074/jbc.M112.444364
PMCID: PMC3724646

Reelin induces EphB activation
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Cell Res. 2013 Apr; 23(4): 473–490. Published online 2013 Jan 15. doi: 10.1038/cr.2013.7
PMCID: PMC3616423

γ-Secretase Mediates Self-Limitation of the Inflammatory Response by Processing LRP1
Kai Zurhove, Chikako Nakajima, Joachim Herz, Hans H. Bock, Petra May
Sci Signal. Author manuscript; available in PMC 2009 Jun 10.Published in final edited form as: Sci Signal. 2008 Nov 25; 1(47): ra15. Published online 2008 Nov 25. doi: 10.1126/scisignal.1164263
PMCID: PMC2694618

Comparative phylogenomic analyses of teleost fish Hox gene clusters: lessons from the cichlid fish Astatotilapia burtoni
Simone Hoegg, Jeffrey L Boore, Jennifer V Kuehl, Axel Meyer
BMC Genomics. 2007; 8: 317. Published online 2007 Sep 10. doi: 10.1186/1471-2164-8-317
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