dUTP solution 100mM
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Delivery time: 3- 8 working days
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Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Deoxyuridine 5'-triphosphate, tetrasodium salt (dUTP) of purity >99% (HPLC). dUTP may be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. Product has been tested for PCR products of length up to 8kb (Taq DNA polymerase) and 0.8kb (Pfu DNA polymerase). The substitution of dTTP for dUTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme Uracil-DNA-Glycosylase, UDG >, can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives.
Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >.
For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our ready-to-use RedMastermix (M3029) >, containing dNTPs already.
Specifications:
Purity: min. 99% (HPLC)
UV-spectrum: confirms
UV-ratios: 250/260nm: 0.74 +/-0.03; 280/260nm: 0.38 +/-0.02; 290/260nm: 0.04 +/-0.01
ε = 9.8 λ x mmol-1 x cm-1
C9H15N2O14P3 (free acid)
MW = 468 g/mol
1mL corresponds to 100 µmol
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
Safety Data SheetProduct description
General Data 1
Source: NCBI PubMed
