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Taq DNA Polymerase 1.1x Master Mix is a ready-to-use 1.1x reaction mix with the Ampliqon Taq DNA polymerase, the NH4+ buffer system, dNTPs and magnesium chloride present. A 50µL PCR reaction requires 45µL of the 1.1x Master Mix. Simply add primers, template and water to a total reaction volume of 50µL to successfully carry out primer extensions and other molecular biology applications. Taq DNA Polymerase 1.1x Master Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
You can find more of our PCR master mixes here.
- High performance thermostable DNA polymerase - Significantly reduced set up time - Diminished risk of contamination - Increased reproducibility - No proofreading – lacks a 3'-5' exonuclease activity - Ideal for TA cloning – leaves an A' overhang
1.1-time ready-to-use PCR Mastermix with 1.5mM MgCl2 (end concentration).
application:PCR master mix for highly convenient PCR
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Taq DNA Polymerase 1.1x Master Mix. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.