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Taq DNA Polymerase 1.1x Master Mix RED is a ready-to-use 1.1x reaction mix with the Ampliqon Taq DNA polymerase, the NH4+ buffer system, dNTPs and magnesium chloride present. Each reaction requires 45μL of the 1.1x Master Mix RED. Simply add primers, template and water to a total reaction volume of 50μL to successfully carry out primer extensions and other molecular biology applications.
Taq DNA Polymerase 1.1x Master Mix RED offers several advantages. Setup time is significantly reduced. The chance of contaminating component stock is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be setup with the confidence that results will be consistent every time. There is no need to buy and use separate loading dyes. Simply load a portion of the PCR reaction product on to an agarose gel for electrophoresis and subsequent visualization. The red dye front runs at 1000 to 2000 bp on a 0.5 to 1.5% agarose gel.
- High performance thermostable DNA polymerase - Significantly reduced set up time - Diminished risk of contamination - Increased reproducibility - No proofreading – lacks a 3'-5' exonuclease activity - Ideal for TA cloning – leaves an A' overhang
1.1-time ready-to-use PCR Mastermix with a red dye and 1.5mM MgCl2 (end concentration).
application:PCR master mix for highly convenient PCR
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-05-02
Dokumente - Protokolle - Downloads
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