IdentityTaq 2X Master Mix for food of animal origin



Order number: M3120.0000

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Direct PCR amplification of meat-or milk-specific DNA sequences from prepared food containing... more
Product information "IdentityTaq 2X Master Mix for food of animal origin"

Direct PCR amplification of meat-or milk-specific DNA sequences from prepared food containing beef, pork, lamb, poultry or turkey, without the need for prior DNA isolation or DNA purification!

The Genaxxon bioscience IdentityTaq 2X Mastermix contains an optimized polymerase that is highly resistant to PCR inhibitors. Fatty samples, such as e.g. Salami, cheese or sausage can be analyzed directly and without prior processing. Other problematic samples, such as wild animal hair or saliva, can thus be easily examined. This master mix is delivered without lysis buffer. If you need the IdentityTaq PCR 2X master mix with lysis buffer, please refer to the M3089 >.

Advantages:
- The IdentityTaq polymerase that is used in our IdentityTaq master mix has been specifically optimized to tolerate a variety of PCR inhibitors that otherwise prevent direct PCR from cell extracts.
- The IdentityTaq 2X master mix ensures reproducible results, and significantly reduces set-up times and the risk of pipetting errors.

NOTE: Due to the use of the specifically engineered Hotstart DNA polymerase and the specific buffer used for the IdentityTaq 2X PCR master mix the use of protocols typically already established in the laboratory is highly likely to result in reaction failure as buffer compositions are different from that used before.

Additional PCR master mixes can be found here: qPCR/realtime master mixes > and Standard PCR master mixes >.

For more information on our product with a direct PCR test for food of animal origin, see our YouTube video:
 watch the video: direct PCR from food of animal origin

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Our comment on "IdentityTaq 2X Master Mix for food of animal origin"
- No need for time-consuming and expensive DNA purification steps - Very little sample material required - Special DNA Polymerase provides high yields of specific product.
Specifications: 2-time master mix for direct PCR of meat-specific DNA sequences from prepared... more
 

Technical Data:

Specifications:
2-time master mix for direct PCR of meat-specific DNA sequences from prepared food (Hamburger, Hotdog, Döner Kebap, etc.).
1.25mL is sufficient for 100 x 25µL PCRs.

1.25mL of 2X master mix

 

 

application:

• Laboratory analyses of livestock food; pet food; meat (cooked, boiled, fresh, stored at +2°C to +8°C or frozen) • Direct gene detection and quantification • Screening / High-throughput PCRs

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

Dokumente - Protokolle - Downloads more


Dokumente - Protokolle - Downloads

Here you will find information and further literature on IdentityTaq 2X Master Mix for food of animal origin. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Due to the use of the specifically engineered Hotstart DNA polymerase and the specific buffer used for the IdentityTaq 2X PCR master mix the use of protocols typically already established in the laboratory is highly likely to result in reaction failure as buffer compositions are different from that used before.

The proprietary lysis buffer is different from that routinely used with Proteinase K, for tissue digestion and subsequent PCR, and the annealing temperatures required for subsequent PCR are also likely to be different. We recommend to make a gradient PCR first!

IdentityTaq Mastermix with Animal Hair:
If there is little source material and the PCR results are weak, the following tips may be helpful:
- Reduce lysis buffer: 100μL instead of 200μL
- increase the number of cycles (up to 45)
- Use more lysate for the batch: 4μL instead of 2μL