AQ97 High Fidelity proofreading Polymerase



Order number: A767501

Shipping: shipped on wet ice, store at -20°C

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.


Product of the month: September 2021

You can find an overview of other proofreading polymerases and mastermixes here.
and in our latest blog on proofreading polymerases.

 

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AQ97 High Fidelity DNA Polymerase is a proofreading enzyme for  robust amplification of DNA... more
Product information "AQ97 High Fidelity proofreading Polymerase"

AQ97 High Fidelity DNA Polymerase is a proofreading enzyme for  robust amplification of DNA targets with low to high GC content and long DNA targets  of 18 kb. The DNA binding domain of this polymerase, ensures excellent high fidelity, long range capacity and also fast amplification results. AQ97 High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.

We recommend to use our AQ97 High Fidelity DNA Polymerase with a capability of amplifying DNA targets up to 18 kb. For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.

AQ97 High Fidelity DNA Polymerase is a novel proofreading DNA polymerase. AQ97 High Fidelity DNA Polymerase is a fused protein complex of DNA polymerase with a processivity-enhancing DNA binding domain. Alongside very fast and robust amplification of complex and long targets, AQ97 High Fidelity DNA Polymerase displays a high fidelity ensuring accurate amplification.

The enzyme is well suited for PCR experiments that require amplification with very low error rates, such as cloning/sub-cloning, NGS applications, SNP analysis and mutagenesis.

Features:

• High Fidelity: > 60x Taq fidelity
• High elongation rate: 10 sec/kb (up to 6000bp per minute)
• Long range amplification: 18 kb for human gDNA
• 5'-3' polymerase acitivity and 3'-5' exonuclease activity.
• generates blund ends.

Comparison figures of fidelity values for AQ97 DNA Polymerase, AccuPol DNA Polymerase, two well-recognized high fidelity DNA polymerases P and Q and Taq DNA Polymerase were determined through NGS-based analysis of nucleotide misincorporation during PCR. Initially, PCR amplification was performed on a ~ 200 bp synthetic DNA target, generating PCR products for each of the tested polymerases (using recommended setup conditions). For details see file ??? (document section)

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Our comment on "AQ97 High Fidelity proofreading Polymerase"
High Fidelity: > 60x Taq DNA Polymerase • High elongation rate: 10 sec/kb • 3’ → 5’ proofreading exonuclease activity. • Good coverage on difficult DNA templates with low to high GC content • Long range capability: 11 kb for gDNA
Specifications: • High Fidelity: >60x Taq DNA Polymerase • High elongation rate: 10 sec/kb... more
 

Technical Data:

Specifications:
• High Fidelity: >60x Taq DNA Polymerase
• High elongation rate: 10 sec/kb
• 3’ → 5’ proofreading exonuclease activity.
• Good coverage on difficult DNA templates with low to high GC content
• Long range capability: 18 kb for gDNA
• delivered as 2 units/µL enyzme solution

application:

PCR for cloning, subcloning, mutagenesis, NGS applications, SNP analysis and mutagenesis

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads more


Dokumente - Protokolle - Downloads

Here you will find information and further literature on AQ97 High Fidelity proofreading Polymerase. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.