PCR Kits - Master Mixes
PCR Master Mixes for Standard, Multiplex > and qPCR >, with and without dye: Genaxxon`s PCR mastermixes are special premixed solutions (ready-to-use), containing polymerase, dNTPs >, MgCl2 and a reaction buffer at optimal concentration. All you need to add is the DNA template and primers.
For routine PCR up to 4 kb length of the amplicon, we recommend our proven PCR Mastermix (2X) >.
One of our top products is the RedMastermix (2X) > with an additive and a red dye for better control of pipetting steps. The subsequent electrophoresis can be driven without the addition of loading buffer. This PCR master mix is characterized by a high sensitivity, which leads to high yields.
For hotstart applications up to 4 kb, we recommend the SuperHot Mastermix (2X) > with chemically modified PCR Taq polymerase.
Genaxxons master mixes for qPCR > are the Green- and Probe- Master mixes, specifically optimized for Roche (LightCycler® 480), Qiagen (rotor-Gene®), Life Technologies (StepOnePlus®) and Applied Biosystems (Mx3005P®). The Green Master mixes have a green, intercalating fluorescent dye. This RealTime master mixes are available with different concentrations of ROX®.
Our PCR master mixes are 2-fold concentrated and shipped in aliquots of each 1.25 mL. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles.
