IdentityTaq Kit 2X Master Mix for direct PCR from food of animal origin
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Direct PCR amplification of meat-or milk-specific DNA sequences from prepared food containing beef, pork, lamb, poultry or turkey, without the need for prior DNA isolation or DNA purification!
The Genaxxon bioscience IdentityTaq 2X Mastermix enables PCR amplification of meat-specific or milk-specific DNA target sequences from liquid or solid food, prepared for human or animal consumption, like Hamburgers, hot dogs, doner kebabs, lasagne or other products that contain (should contain) meat or milk products. It requires only a short and easy pre-PCR lysis step without the need for prior DNA isolation and purification. The food can be raw or cooked, non-refrigerated, chilled or frozen. Simply take a 50mg - 100mg sample of food containing the meat and add 200μL lysis buffer. After the lysis step, the PCR can be started immediately. The IdentityTaq 2X master mix ensures reproducible results and significantly reduces set-up times and the risk of pipetting errors.
The IdentityTaq polymerase, which is used in our IdentityTaq master mix, has been specifically optimized to tolerate a variety of PCR inhibitors that otherwise prevent direct PCR from cell extracts.
NOTE: The accompanying protocol was developed for specific use with the engineered HotStart DNA polymerase, and the buffer contained in the IdentityTaq 2X PCR Master Mix.
Due to the use of the specifically engineered HotStart DNA polymerase and the specific buffer used for the IdentityTaq 2X PCR Master Mix, the use of protocols typically already established in the laboratory is highly likely to result in reaction failure as buffer compositions are different from that used before.
The proprietary lysis buffer is different from that routinely used with Proteinase K, for tissue digestion and subsequent PCR, and the annealing temperatures required for subsequent PCR are also likely to be different.
Additional PCR master mixes can be found here: qPCR/realtime master mixes > and Standard PCR master mixes >.
Specifications:
2-time master mix for direct PCR of meat-specific DNA sequences from prepared food (Hamburger, Hotdog, Döner Kebap, etc.).
Supplied with a separate lysis buffer and according to customers´ demand also with a positive control DNA and species specific primers.
1mL is sufficient for 80 x 25µL PCRs.
1mL of 2X master mix with 22mL separate lysis buffer.
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
ManualsGeneral Data 3
Source: NCBI PubMed
Due to the use of the specifically engineered Hotstart DNA polymerase and the specific buffer used for the IdentityTaq 2X PCR master mix the use of protocols typically already established in the laboratory is highly likely to result in reaction failure as buffer compositions are different from that used before.
The proprietary lysis buffer is different from that routinely used with Proteinase K, for tissue digestion and subsequent PCR, and the annealing temperatures required for subsequent PCR are also likely to be different. We recommend to make a gradient PCR first!
IdentityTaq Mastermix with e.g. hair from animals:
If there is little source material and the PCR results are weak, the following tips may be helpful:
- Reduce lysis buffer: 100μL instead of 200μL
- increase the number of cycles (up to 45)
- Use more lysate for the batch: 4μL instead of 2μL
