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Oligo (dT)20 are single stranded oligodeoxyribonucleotides containing only deoxythymine (dT) to be able to prime with the poly(A) tail of mRNA molecules. Primers are quality controlled (MS), purified (reversed phase HPLC), aliquoted and lyophilized and came with a guaranteed amount of at least 5OD (about 27.2nmol (163.7µg)).
Oligo (dT)20 Primer is designed to initiate the synthesis of cDNA from total RNA in a reverse transcription reaction, where Reverse Transcriptase, e.g. MMuLV (M3042) > is starting the reaction from the poly-A-end of mRNA. It can also be used for generation of labeled cDNA to screen microarrays.
A mixture (1:1 ratio) of random hexamer primers (M3038 >) and Oligo(dT)20 primer may improve the sensitivity of cDNA synthesis as it will especially improve the efficiency of transcripts longer than 600 bp.
Guaranteed delivery of at least 5OD (equals about 27.2nmol (163.7µg)). Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted. Dissolve content (5 OD) in 271.5μL of molecular biology grade water for an end concentration of 100 pmol/μL (100μM). 760μL (100μM) are sufficient for 760 reverse transcriptions of 20μL each.
Oligo (dT)20 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase, such as MMuLV or AMV. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)20 ensures that the 3´ end of mRNAs are represented.
Oligo(dT)20 Primer may be better suited for the new generation of Reverse Transcriptases like SuperScript™ that work at higher temperatures as the longer Oligo(dT)20 Primer enable annealing in reverse transcription reactions at higher temperatures.
• cDNA synthesis from total RNA in a reverse transcription reaction.
• optimal choice for construction of cDNA libraries from eukaryotic mRNAs.
• Full length cDNA cloning
• 3’ rapid amplification of cDNA ends (3’ RACE)
• Generation of labelled cDNA for screening of microarrays.
Oligo (dT)20 Primer can not be used together with degraded RNA, prokaryotic RNA or miRNA (lack of poly(A) tail).
Primer Sequence: 5´ – d (TTT TTT TTT TTT TTT TTT TT) –3´
Amount: 27.2nmol / 163.7µg / 5 OD
Purification: desalted (HPLC purified and MS checked)
Primer sequence: 5'd PO4 [(T)20] 3'
5' Primer modification: Phosphate
application:Oligo(dT)-Primer will anneal to the polyA tail of RNA to serve as primers for cDNA synthesis by a reverse transcriptase.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 31-38-01-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Oligo dT20 primer. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.