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Uracil DNA Glycosylase (UDG), also called uracil N-glycosylase (UNG), catalyzes the release of uracil from uracil-containing DNA. The UDG/UNG from Genaxxon is a highly purified, recombinant enzyme isolated from E. coli with a mass of 26 kDa. UDG efficiently hydrolyzes single- and double-stranded DNA containing uracil, except for oligomers with 6 or fewer bases. Therefore, uracil-DNA glycosylase (UDG) is used wherever it is necessary to avoid the risk of DNA carryover (contamination) during PCR.
In order to be able to hydrolyze DNA by UDG/UNG you do need dUTP > in your PCR reactions.
- 20mM Tris
- HCl (pH 8.0)
- 50mM NaCl
- 0.1mM EDTA
- 50% Glycerin
Reaction buffer (1X):
- 20 mM Tris
- HCl (pH 8.0)
- 1 mM EDTA
- UDG / UDG prevents from carryover contamination PCR products and thus avoids false positive results. - No interference with any PCR- and realtime PCR application.
Activity: 10 units/µL.
delivered in storage buffer: 20mM Tris, HCl (pH 8.0), 50mM NaCl, 0.1mM EDTA, 50% glycerol
application:for prevention of carry over of DNA in PCR
Unit Definition:One unit of activity is defined as the amount of enzyme required to release 60 pmol of uracil per minute from double-stranded, uracil containing DNA.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Uracil-DNA Glycosylase (UDG). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.