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Green DNA Dye is a sensitive fluorescent dye for detection of dsDNA in qPCR (real-time PCR) and other applications. Because Green DNA Dye has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence Green DNA Dye is ideal for use with laser scanners. When Green DNA Dye is free in solution, it emits a very low fluorescent signal. As soon as the dye binds to the double-stranded DNA, the signal increases significantly (thousand fold), which makes the fluorescent signal of the dye directly proportional to the amount of amplified dsDNA.
Green DNA Dye is delivered as 100-time solution in DMSO. The concentration of Green DNA Dye is traditionally given as a dilution factor where the 1X dilution is used for staining of DNA gels. For PCR, a 0.2X dilution is the starting dilution for optimization. In the detection of double-stranded DNA in native polyacrylamide gels it is also much more sensitive than silver staining.
Preparation of a working solution for realtime PCR reactions:
Green DNA Dye is suitable for real-time PCR with a suggested concentration for short targets of 0.2X (short targets: 50 – 400bp).
Dilute delivered 100-time stock solution with nuclease-free PCR grade water by 1:10 (pre-dilution) and add 0.4µL of this solution to a 20µL final volume (PCR reaction). The 10-time working solution has to be kept at -20°C and has to be renewed every 2-3 weeks latest.
Low toxic fluorescent nucleic acid dye as an alternative to ethidium bromide: GelRed® >.
4-5 times more sensitive than Ethidium bromide
100-time stock solution in DMSO.
application:Fluorescence dye for staining of DNA Fluorescence dye for realtime detection of DNA in qPCR reactions
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-03
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Green DNA Dye - 300 µL. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
The occurrence and formation of monoterpenes in herbivore-damaged poplar roots
Nathalie D. Lackus, Sandra Lackner, Jonathan Gershenzon, Sybille B. Unsicker, Tobias G. Köllner
Sci Rep. 2018; 8: 17936. Published online 2018 Dec 18. doi: 10.1038/s41598-018-36302-6