Order number: M3071.0025Shipping: shipped and stored at RT
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Delivery time 1-3 workdays
Lyophilized (freeze-dried) temperature stable and pre-mixed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications.
No cooling/cooling chain necessary! Our LyoMix and all ingredients are unopened stable at ambient temperature for at least 24 months!
Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.
This lyophilized master mix in powder form is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add PCR-grade water, template and primers to reconstitute the master mix and you are all set!
Test sample available at a special price! The test sample price will be refunded on the first official order of the product.
Lyophilized realtime PCR master mix:
• Hotstart Polymerase
• increased specificity
• increased sensitivity
• for difficult to amplify templates with high GC content
• for Multiplex applications
• for qPCR
This lyophilized Hotstart master mix is delivered in powder form that can be easily dispensed.
We also offer our SuperHot Hotstart Master Mix > with and without dye.
With our high quality dNTPs as Set > (M3015.4100 and M3015.0250) or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.
- freeze-dried realtime PCR reagents mix in powder format. No need for freezing anymore! - just dilute with PCR-grade water to get your 2-time realtime PCR master mix. - long-term ambient-temperature stable
lyophilized master mix for 100 x 20µL qPCR reactions (M3071.0100) or 500 x 20µL qPCR reactions (M3071.0500).
LyoMix is a ready-to-use freeze-dried master mix that contains all necessary components for realtime PCR.
This master mix is stable for at least 24 months at ambient temperature if the supplied tube is kept unopened!
application:For multiplex PCR. For Real-time PCR. For PCR of low amount of DNA (low copy number).
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on LyoMix - Lyophilized qPCR probe master mix powder. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.