Order number: M3074.0000Shipping: shipped and stored at RT. Separate buffer has to be stored at -20°C
Delivery time 14 Workdays
The qPCR Green LyoMix HRM Master Mix is a lyophilized, temperature stable, premixed and pre-portioned Hotstart master mix with all necessary components optimized for e.g. highly specific qPCR or post-PCR high-resolution melt analysis (HRM).
The lyophilized mix contains Hotstart Taq DNA polymerase, dNTPs, MgCl2, green fluorescent dye, enhancers and stabilizers in a proprietary formulation that enables optimized HRM applications (SNP genotyping, mutant scanning and methylation recognition).
No cooling/cooling chain necessary! Our LyoMix products and all ingredients are unopened stable at ambient temperature for at least 24 months!
Our lyophilized qPCR master mix is also available as LyoBalls > pre-portioned in PCR tube strips (0.1ml or 0.2mL tubes) or in PCR plates (0.1mL or 0.2mL wells)!
Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.
This lyophilized master mix in powder form is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add PCR-grade water, template and primers to reconstitute the master mix and you are all set!
Test sample available at a special price! The test sample price will be refunded on the first official order of the product.
Lyophilized realtime PCR master mix:
• Hotstart Polymerase
• increased specificity
• increased sensitivity
• optimized for HRM applications (SNP, mutation scanning)
• for qPCR
We also offer our SuperHot Hotstart Master Mix > with and without dye.
- freeze-dried realtime PCR reagents mix in powder format. No need for freezing anymore! - just dilute with dilution buffer to get your 2-time realtime PCR master mix. - long-term ambient-temperature stable
lyophilized master mix for 100 x 20µL qPCR reactions (M3074.0100) or 500 x 20µL qPCR reactions (M3074.0500).
LyoMix is a ready-to-use freeze-dried master mix that contains all necessary components for realtime PCR.
This master mix is stable for at least 24 months at ambient temperature if the supplied tube is kept unopened!
application:For SNP analytics. For Real-time PCR. For PCR of low amount of DNA (low copy number).
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on qPCR Green LyoMix HRM - No ROX - lyophilized qPCR master mix. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.