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5X Multiplex PCR Mastermix for robust PCR with all components for rapid, sensitive and reproducible quantification of DNA. The optimized DNA polymerase and an optimized buffer including our ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup. Our 5X qPCR master mix will function with a wide range of templates including human-, mammal-, and plant-derived samples.
- amplification of different targets in a single PCR tube (tested for up to 4 targets).
- contains an optimized DNA polymerase
- the hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup
- the optimized buffer includes our ultrapure dNTPs
Our new lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy.
Our new 5-fold multiplex mastermix minimizes the need for optimization and therefore makes the development and establishment of multiplex PCR easier and faster.
realtime master mixes for your realtime PCR experiments can be found here >.
Examples of Multiplex applications:
F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002.
Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.
This multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy. - Amplification of multiple targets in a single tube (tested for up to 4 targets) - Fast qPCR due to minimized denaturation times
5-time ready-to-use multiplex master mix with an optimized modified Taq DNA polymerase.
application:Multiplex PCR for Microsatellite analysis clonality analysis gene rearrangement analysis
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34160490
Dokumente - Protokolle - Downloads
Here you will find information and further literature on 5X qPCR Multiplex MasterMix. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.