M-MuLV Reverse Transcriptase RNase H negative
Order number: M3042.1010
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The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. M-MuLV RT will also extend primers hybridized to single-stranded DNA!
The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.
Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
Genaxxon offers the enzyme HotScriptase (M3056) > and the HotScriptase master mixes (M3062 >; M3064 >) with which it is possible to synthesize double stranded DNA completely without an isothermal transcription step. Time saving: up to 50%! No need for Superscript!
Money saving: high!
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
Free of RNase activity resulting in an increase of full-length cDNA products. M-MuLV is used in cDNA synthesis with long templates.
Technical Data:
Specifications:
Activity: 200 - 400 CEL units/µL.
Purity: Free of RNase activity resulting in an increase of full-length cDNA products.
Purity: >98% protein homogeneity in 10% SDS-PAGE electrophoresis.
MW = 69 kDa.
Enzyme is shipped together with complete buffer.
application:
reverse transcription cDNA synthesisSource
E.coliSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 34-16-04-90Dokumente - Protokolle - Downloads
Here you will find information and further literature on M-MuLV Reverse Transcriptase RNase H negative. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
