HotScriptase Probe RT-qPCR master mix without ROX



Order number: M3064.0000

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HotScriptase Probe master mix for RT-PCR without an isothermal intermediate step, thereby saving... more
Product information "HotScriptase Probe RT-qPCR master mix without ROX"

HotScriptase Probe master mix for RT-PCR without an isothermal intermediate step, thereby saving time and money: HotScriptase Probe master mix was successfully used by several customers for the detection of RNA viruses like Sika or SARS-CoV-2 (COVID-19 detection). HotScriptase is a extremely thermostable reverse transcriptase and DNA polymerase in one enzyme.

HotScriptase Probe master mix enables the amplification of RNA or DNA target sequences with quick and easy PCR protocols, even without an isothermal reverse transcription step. The light blue color improves the visibility of the mixture during the pipetting steps, especially if white or transparent PCR plates or PCR tubes are used. (find out more about our realtime master mixes >)

  • faster: One-Step - NO time consuming isothermal intermediate step necessary.
  • easier: reverse transcriptase and DNA polymerase in one single enzyme; reverse transcription and DNA amplification in parallel during the PCR elongation step. You need only a PCR protocol.
  • saver: much reduced contamination risk, due to reduced pipetting steps.
  • extreme thermostablility: secondary structures will be a minor problem due to higher temperatures of PCR compared to reverse transcription reactions.
  • cheaper: parallel reverse transcription and amplification by HotScriptase RT saves time and costs.

The Genaxxon bioscience HotScriptase can be ordered as HotScriptase DNA polymerase > with 5 units/µL or as a convenient 2-time RT master mix. The 2-time RT master mix contains all necessary ingredients for RT-PCR without specific primers, probes or RNA/DNA. Additionally 

The Genaxxon HotScriptase DNA polymerase > for reverse transcription enables a "One-Step” RT-PCR directly from cell suspensions or mammals cells without an isothermal reverse transcription intermediate step. Just give cells or cell suspension (100 up to <10,000 cells) directly to the PCR reaction mixture, mix, place in a thermal cycler and start PCR program.

As there is no need for expensive and time consuming cell lysis, isothermal transcription or RNA purification, the Genaxxon HotScriptase Cell RT master mix > is ideally suited for High-throughput applications as RNAi knockdown, gene expression studies or realtime PCR (qPCR). The ready-to-use 2-time master mix ensures reproducible RT-PCR results in addition to very much reduced setup times and the reduced risk of contamination due to pipetting errors.

HotScriptase RT polymerase is a biotechnical engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution. The HotScriptase 2-time master mix is a ready-to-use mixture of the propietory HotScriptase, buffer, dNTPs and special components. The recommended amplicon size should be between 60-400 bp!

This master mix contains no ROX nor green dye!

> find out about our realtime master mixes
> troubleshooting & complaints feeedback form
> frequently asked questions (FAQs)

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Our comment on "HotScriptase Probe RT-qPCR master mix without ROX"
RT-PCR possible without reverse transcription step A sample extraction and sample lysis is not necessary as immediate hostart PCR cycling conditions can brake cell- and virus membranes (starting with an at least 3 minutes initial 95°C denaturation and lysis step)
Specifications: 2-time PCR and RT-PCR mastermix with "fast start function" with high quality... more
 

Technical Data:

Specifications:
2-time PCR and RT-PCR mastermix with "fast start function"
with high quality dNTPs
without green fluorescence dye
without ROX as passive reference dye

application:

RNA transcription qPCR primer extension of RNA or DNA

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
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Dokumente - Protokolle - Downloads

Here you will find information and further literature on HotScriptase Probe RT-qPCR master mix without ROX. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
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Dokumente - Protokolle - Downloads

HotScriptase is only working with specific primers! We highly recommend to design primers with an annealing temperature >65°C.

Our HotScriptase may fail if you use your normally designed primers for RT-PCR.

optimized RT-qPCR protocol in using HotScriptase for Covid-19 detection

70°C 5 minutes
93°C 3 seconds
68°C 2.5 minutes
93°C 3 seconds
66°C 2.5 minutes

95°C 10 seconds
57°C 15 seconds
72°C 15 seconds (40-45 cycles)
< 10°C hold