Order number: M3068.0100Shipping: shipped on wet ice, store at -20°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Our cDNA RT-PCR One-Step Kit is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse transcriptase with enhanced thermal stability resulting in an increased specificity, high cDNA yield and an improved efficiency for highly structured and long cDNA fragments.
Besides the reverse transcriptase the enzyme mixture contains our HotStart Polymerase and RNase Inhibitor in 50% glycerol (v/v). The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffers ensure superior amplification results.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.
In one-step RT-PCR all components of reverse transcription (RT) and PCR are mixed in one tube prior to starting the reaction and thus carried out sequentially without opening the tube. This offers tremendous convenience when applied to analysis of single targets from multiple samples of RNA and minimizes the risk of contaminations.
Genaxxon offers also a “Zero-Step” HotScriptase RT (M3056) a RNA/DNA Polymerase that enables direct PCR from RNA without an isothermal reverse transcription step. Just starting the PCR protocol with specific primers. Especially suited for RNA targets that exhibit a high degree of secondary structure, for self- or cross-complementary primers.
Genaxxon offers the enzyme HotScriptase (M3056) > and the HotScriptase master mixes (M3062 >, M3064 >) with which it is possible to synthesize double stranded DNA completely without an isothermal transcription step. Time saving: up to 50%! No need for Superscript!
Money saving: high!
One-Step RT-PCR long cDNA templates
Ready-to-use enzyme mixture for One-Step RT-PCR containing reverse transcriptase, hotstart polymerase and RNAse Inhibitor in 50% glycerol (v/v).
2-time RT-PCR buffer including ultra-pure dNTPs.
Free of RNase activity resulting in an increase of full-length cDNA products.
application:reverse transcription cDNA synthesis
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on One-Step cDNA RT-PCR Kit. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.