One-Step RT-qPCR 2X GreenMastermix



Order number: M3137.0250

Shipping: Shipment: on wet ice. Store at -20°C. For laboratory usage only!

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€479.54 *

Volume:

Please select the favoured pack size.

Our One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA... more
Product information "One-Step RT-qPCR 2X GreenMastermix"

Our One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates using EvaGreen®. The ready-to-use mixture is based on a genetically modified reverse transcriptase with improved thermal stability, which offers increased specificity, a high cDNA yield and improved efficiency for highly structured and long cDNA fragments. As a further improvement, EveGreen® was used as the fluorescent dye, which inhibits PCR less than SYBR® Green.

The 2X Mastermix contains all the reagents required for the RT-qPCR (except for the template, primer and probes) to ensure quick and easy handling at a minimum of pipetting steps. The high-quality enzymes and the optimized reaction buffer with high-purity dNTPs guarantee superior real-time PCR results.

RT-qPCR is used to amplify double-stranded DNA from single-stranded RNA templates to enable rapid real-time quantification of RNA targets. In the reverse transcription step, the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA). The cDNA molecules are amplified by the HotStart DNA polymerase. The HotStart polymerase activity is blocked at ambient temperature and will be activated at the beginning of the initial denaturation. This thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during the PCR setup.

One-Step RT-qPCR offers enormous convenience when analyzing targets from multiple RNA samples and minimizes the risk of contamination.

The cDNA One-Step RT-qPCR 2X master mix is also available as a variant without EvaGreen® for probe based qPCR (M3138>).

Genaxxon offers the enzyme HotScriptase (M3056) > and the HotScriptase master mixes (M3062 >, M3064 >) with which it is possible to synthesize double stranded DNA completely without an isothermal transcription step. Time saving: up to 50%! No need for Superscript!
Money saving: high!

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One-Step RT-qPCR long cDNA templates
Specifications: Ready-to-use 2-time RT-qPCR mix with EvaGreen® with Reverse Transcriptase with... more
 

Technical Data:

Specifications:
Ready-to-use 2-time RT-qPCR mix with EvaGreen®
with Reverse Transcriptase
with Hot Start Polymerase
with RNase Inhibitor
with dNTPs

application:

reverse transcription cDNA synthesis

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
Dokumente - Protokolle - Downloads more


Dokumente - Protokolle - Downloads

Here you will find information and further literature on One-Step RT-qPCR 2X GreenMastermix. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.