Order number: M3134.0020
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
The Genaxxon bioscience Scriptase RT – cDNA Synthesis Kit contains all reagents required for first strand cDNA synthesis in one box combining simple handling with high flexibility. The premium quality Reverse Transcriptase, ultrapure dNTPs and an optimized reaction buffer ensure superior results with highest reproducibility. The kit is optimized for high efficiency in a broad range of primer-template combinations, also for small amounts of RNA.
It contains all reagents required for cDNA synthesis (except template and specific primers) combining simple handling with high flexibility. The high quality Scriptase Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability, ultrapure dNTPs and the optimized complete reaction buffers ensure superior amplification results.
Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT.
The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.
for cDNA from 100bp up to 19kbp
Scriptase RT: 200 units/µL in storage buffer containing 50% glycerol (v/v).
5X RT Reaction Buffer: 250mM Tris/HCl (pH8.3), 375mM KCl, 15mM MgCl2, 50mM DTT
dNTP-Mix 10 mM each dNTP (dATP, dCTP, dGTP, dTTP)
DTT stock solution 100mM
Oligo-(dT)20 primer 100µM
Random Hexamers 100µM
Positive Control RNA 10ng/µL
RNase Inhibitor 40 units/μL RNase inhibitor in storage buffer with 50% glycerol (v/v)
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
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