Lysozyme from chicken egg, ca. 20000 U/mg



Order number: S5237.0005

Shipping: shipped at RT, store at -20°C
CAS-Nr: 12650-88-3, 9001-63-2

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€68.50 *

Weight:

Please select the favoured pack size.

The enzyme Lysozyme (Muramidase) affects the cell walls of bacteria. Thereby the extraction... more
Product information "Lysozyme from chicken egg, ca. 20000 U/mg"

The enzyme Lysozyme (Muramidase) affects the cell walls of bacteria. Thereby the extraction efficiency of proteins or nucleic acids is significantly improved. Genaxxon Lysozyme from chicken egg is available as lyophilized powder.

Lysozyme preferentially hydrolyses the β-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganisms. The enzyme is present in many organisms. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. The lysis of E.coli is improved by the combined addition of both lysozyme and and a nuclease such as DNase I (M3028) >. The working concentration is 200-300 μg/µL. To increase the plasmid yield (approx. 5-10%), lysozyme may be added. Another application is the lysis of bacteria for the preparation of bacterial RNA. In this case the working concentration is 40μg/mL (stock solution 50mg/mL).

Lysozyme from chicken egg is most effective for the lysis of gram-positive bacteria. Besides this, it facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella.

  • Lyophilized, white powder
  • Enzyme for lysis of bacterial cell walls
  • Improvement of protein purification from inclusion bodies
  • Activity: min. 20.000 U/mg (pH: 6.25)

Lysozyme will be inhibited by surfactants like SDS (M3292) > or alcohols and fatty acids, Imidazole (M6033) > and Indol-derivatives.

Stability: The lyophilized powder of lysozyme is stable for many years at +2°C to +8°C. In solution, the stability at pH values from 4 to 5 at +2°C to +8°C is several weeks and at room temperature several days. pH-optimum is at 9.2, the isoelectric point at 11.0.

More products around "Lysozyme from chicken egg, ca. 20000 U/mg"
Our comment on "Lysozyme from chicken egg, ca. 20000 U/mg"
albumin free salt free
Specifications: Activity: min. 20,000 U/mg (pH6.25) Appearance: white powder, ... more
 

Technical Data:

Specifications:
Activity: min. 20,000 U/mg (pH6.25)
Appearance: white powder, albumin-free, freeze-dried
MW: approx. 14,400 g/mol
Stability (lyophilised and stored at -20°C): several years / (in solution at +2°C to +8°C): several weeks

application:

Plasmid isolation RNA isolation protoplasts generation

Source

from chicken egg white

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

EC-Nr: 236-747-3
CAS-Nr: 12650-88-3, 9001-63-2
eclass-Nr: 34-16-05-22
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on Lysozyme from chicken egg, ca. 20000 U/mg. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.