Lysozyme from chicken egg white, min. 20000 U/mg - Molbio Grade
Order number: S5053.0001
Shipping: Shipment: not cooled. Store at -20°C. For laboratory usage only!CAS-Nr: 9001-63-2
Delivery time: 3- 8 working days
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Lysozyme (Muramidase) preferentially hydrolyses the β-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganisms. The enzyme is present in many organisms. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. The working concentration is 200-300 μg/µL. To increase the plasmid yield (approx. 5-10%) lysozyme may be added. Another application is the lysis of bacteria for the preparation of bacterial RNA. In this case the working concentration is 40μg/mL (stock solution 50mg/mL).
Stability: The lyophilized powder of lysozyme is stable for many years at +2°C to +8°C. In solution, the stability at pH values from 4 to 5 at +2°C to +8°C is several weeks and at room temperature several days. pH-optimum is at 9.2, the isoelectric point at 11.0.
Lysozyme will be inhibited by surfactants like SDS or alcohols and fatty acids, imidazole and indol-derivatives.
Technical Data:
Specifications:
Activity: min. 20,000 U/mg (pH6.2)
Appearance: white powder, albumin-free, freeze-dried
MW: approx. 14,400 g/mol
Stability (lyophilised and stored at -20°C): several years / (in solution at +2°C to +8°C): several weeks
DNAse and RNAse free.
application:
Plasmid isolation RNA isolation protoplasts generationSource
from chicken egg whiteSicherheits Hinweise / Safety
Klassifizierungen / Classification
EC-Nr: 232-620-4CAS-Nr: 9001-63-2
eclass-Nr: 32-16-04-10
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Lysozyme from chicken egg white, min. 20000 U/mg - Molbio Grade. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
