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Hyaluronidase isolated from sheep testes. The mammalian glycolytic hyaluronidase (EC 188.8.131.52) catalyzes the hydrolysis of the 1-4 bond between N-acetyl-D-glucosamine and D-glucuronic acid in hyaluronic acid. It also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin sulfates A and C, and dermatan. In opposite to S5234, S5232 shows a higher activity of 500 U/mg substance.
pH-Optimum of the enzyme: 7.0-8.0.
Stock solution: 1-300mg/mL in phosphate buffer or water.
Working solution: 0.1-10mg/mL.
Synonyms: Hyaluronoglucosidase, Hyaluronate 4-glycanohydrolase, Mucinase.
Assay: min. 500 U/mg
Appearance: lyophilized salt free powder
MW = ca. 70 kDa.
application:for degradation of Hyaluronic acid and Chondroitin sulfate in extracellular matrices.
Unit Definition:One unit is defined as the amount of enzyme which causes a reduction in turbidity the same as the International Unit (I.U.) as compared to the International Standard.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 253-464-3
Dokumente - Protokolle - Downloads
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