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Lens culinaris lectin or agglutinin (LCA) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 46 kDa and it forms a complex together with sucrose. Lectins are, due to their specific binding to carbohydrate structures on the cell surface or elsewhere useful in haematology, immunology or as specific markers for membrane glycoprotein structures. LCA's carbohydrate specificity is D-mannose and D-glucose and the two isomers LCA-A and LCA-B agglutinates human red blood cells, although this reaction is not blood group specific. LCA is also a useful component in affinity chromatography columns for the separation of glycoconjugates. Adding 60mM methylmannoside yields an inhibition with a lectin titer that is at least 16-fold weaker than the control. Addition of Mn2+ and Ca2+ to the reconstitution buffer will enhance hemagglutination activity.
Appearence White lyophilized powder or flocculate
Assay: protein content: min. 85% (OD280nm (e 1mg/mL = 1.34)).
Identity: Isoelectric focusing, Phast gel IEF. Two major bands at pI 8.15, 8.45 and one minor at pI 8.65.
Source: Lentil seeds
Sugar specificity: α-D-mannose and α-D-glucose
Activity: Agglutinates human erythrocytes in a 2% blood suspension with lectin concentration ≤8µg/mL in 0.9% NaCl after 2 h at 25°C.
Microorganisms: <100 CFU/g
Molecular weight 46 kDa
application:- Haemagglutination studies - Cell agglutination studies - Component in affinity columns - Separation of glycoconjugates
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
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