Phaseolus vulgaris lectin P

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. The lectin consists of the two subunits lectins PHA-E (erythroagglutinin) and PHA-L (leucoagglutinin) thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 115 kDa and specificity for complex oligosaccharides. PHA-P is not blood group specific. PHA-P lectin is supplied as a white to light pink lyophilized powder from a buffer containing 50mM NH4HCO3. The purity of the lectin is determined by SDS-PAGE generating one single band. PHA-P is available in vials containing 5mg or 10mg lyophilized powder and the product is to be used for laboratory work only.