Lipobiotin - PHC-KKKKK(Biotin-Aca-Aca)

Lipobiotin - PHC-KKKKK(Biotin-Aca-Aca) is a selectively biotinylated analogue of PHC-SKKKK (P2232 >). Biotin is attached via spacer molecules to the side chain of the C-terminal lysine. The Biotin-avidin or -streptavidin interaction can be used for detection, labelling or immobilisation in many research applications. Lipobiotin is no ligand of TLR. Lipobiotin was used in a novel, rapid and versatile method for the isolation of pathogen-containing organ-elles from primary cells. The compound was used to functionalize bacterial surfaces and allows the rapid immunomagnetic isolation of intteria-containing compartments and apoptotic blebs which can be characterized by electronmicroscopy, western blot and mass spectrometry. The procedure facilitates the detailed molecular characterization of pathogen-containing phagosomes in low total cell number of macrophages and other host cells.

References:
C. Steinhäuser, U. Heigl, V. Tchikov, J. Schwarz, T. Gutsmann, K. Seeger, J. Brandenburg, J. Fritsch, J. Schroeder, K.-H. Wiesmüller, I. Rosenkrands, P. Walther, J. Pott, E. Krause, S. Ehlers, W. Schneider-Brachert, S. Schütze, N. Reiling (2013) Lipid-labeling facilitates a novel magnetic isolation procedure to characterize pathogen-containing phagosomes. Traffic 14(3), 321-336. Doi: 10.1111/tra.12031.
C. Steinhäuser, T. Dallenga, V. Tchikov, U. E. Schaible, S. Schütze, N. Reiling (2014) Immuno-magnetic isolation of pathogen-containing phagosomes and apoptotic blebs from primary phagocytes. Curr. Protoc. Immunol., 2014 Apr 2;105:14.36.1-26. doi: 10.1002/0471142735.im1436s105.