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Lipobiotin - PHC-KKKKK(Biotin-Aca-Aca) is a selectively biotinylated analogue of PHC-SKKKK (P2232 >). Biotin is attached via spacer molecules to the side chain of the C-terminal lysine. The Biotin-avidin or -streptavidin interaction can be used for detection, labelling or immobilisation in many research applications. Lipobiotin is no ligand of TLR. Lipobiotin was used in a novel, rapid and versatile method for the isolation of pathogen-containing organ-elles from primary cells. The compound was used to functionalize bacterial surfaces and allows the rapid immunomagnetic isolation of intteria-containing compartments and apoptotic blebs which can be characterized by electronmicroscopy, western blot and mass spectrometry. The procedure facilitates the detailed molecular characterization of pathogen-containing phagosomes in low total cell number of macrophages and other host cells.
C. Steinhäuser, U. Heigl, V. Tchikov, J. Schwarz, T. Gutsmann, K. Seeger, J. Brandenburg, J. Fritsch, J. Schroeder, K.-H. Wiesmüller, I. Rosenkrands, P. Walther, J. Pott, E. Krause, S. Ehlers, W. Schneider-Brachert, S. Schütze, N. Reiling (2013) Lipid-labeling facilitates a novel magnetic isolation procedure to characterize pathogen-containing phagosomes. Traffic 14(3), 321-336. Doi: 10.1111/tra.12031.
C. Steinhäuser, T. Dallenga, V. Tchikov, U. E. Schaible, S. Schütze, N. Reiling (2014) Immuno-magnetic isolation of pathogen-containing phagosomes and apoptotic blebs from primary phagocytes. Curr. Protoc. Immunol., 2014 Apr 2;105:14.36.1-26. doi: 10.1002/0471142735.im1436s105.
Lipopeptides are very hydrophobic peptides. For this reason it is most often not possible to purify them by HPLC and to give reliable purity values (in %) for our standard lipopeptides.
MW = 2018 g/mol + 228 g/mol (TFA)
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
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