alpha-Chymotrypsin (EC 3.4.21.1)
Order number: S5228.0001
Shipping: Shipment: not cooled. Stored at -20°C. For laboratory usage only!CAS-Nr: 9004-07-3
Delivery time: 3- 8 working days
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alpha-Chymotrypsin is a serine peptidase that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Leu) on the carboxyl end of the bond. Ca2+ activates and stabilizes the enzyme. The enzyme has 241 amino acid residues in total contained in three polypeptide chains (A chain: 13 amino acids; B chain: 131 amino acids, and C chain: 97 amino acids) linked by disulfide bridges.
alpha-Chymotrypsin is inhibited by diisopropyl fluorophosphate (DFP) >, phenylmethanesulfonyl fluoride (PMSF) >, N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK) >, chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10mM Cu2+ and Hg2+.
Technical Data:
Specifications:
Activity: min. 400 U/mg
Appearance: off-white powder
pH: 4.0 - 5.0
Loss on drying: max. 5%
Specific absorbance (280nm): min. 18.5, max. 22.5
Specific absorbance (250nm): max. 8.0
MW = about 25,000 g/mol
application:
digestion of proteins/peptidesUnit Definition:
One unit is defined as the amount of enzyme that causes a decrease in absorbance at 237nm of 0.0075 per minute at 25°C resulting from the hydrolysis of ATEE (NF/USP unit).Source
bovine pancreasSicherheits Hinweise / Safety
H-Sätze: H315, H319, H334, H335GHS-Symbole: GHS07, GHS08
R-Sätze: R36/37/37-42
Gefahrstoffkürzel: Xn
Klassifizierungen / Classification
EC-Nr: 232-671-2CAS-Nr: 9004-07-3
eclass-Nr: 32-16-04-10
Dokumente - Protokolle - Downloads
Here you will find information and further literature on alpha-Chymotrypsin (EC 3.4.21.1). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
