Order number: S5390.0001Shipping: shipped at RT, store at +2°C to +8°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.
Protein binding capacity: 70mg/mL settled beads
Metal capacity: >12µeqv Ni2+/mL
Possible metal ions: Co2+, Zn2*, Fe3+, Al3+ and Cu2+
Microbial preservative: 20% ethanol
Delivered as a 25% suspension (1mL suspension will result in 250µL gel bed volume). Suspension contains 20% ethanol as a microbial preservative.
application:Purification of recombinant Proteins carrying a polyhistidine tag
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-02
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Ni-NTA MagBeads for His-tagged protein purification. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Timothy Alexander Hore, Ferdinand von Meyenn, Mirunalini Ravichandran, Martin Bachman, Gabriella Ficz, David Oxley, Fátima Santos, Shankar Balasubramanian, Tomasz P. Jurkowski, Wolf Reik
Proc Natl Acad Sci U S A. 2016 Oct 25; 113(43): 12202–12207. Published online 2016 Oct 11. doi: 10.1073/pnas.1608679113
Pavel Bashtrykov, Gytis Jankevicius, Renata Z. Jurkowska, Sergey Ragozin, Albert Jeltsch
J Biol Chem. 2014 Feb 14; 289(7): 4106–4115. Published online 2013 Dec 24. doi: 10.1074/jbc.M113.528893