Affinity Chromatography Purification

Affinity chromatography is a useful technique for separating proteins in a reversible interaction between the target protein and ligands bound to beads (affinity resin). The interaction can be biospecific, for example antibodies binding to protein A, or non-biospecific, for example histidine-tagged proteins binding to metal ions.

The chromatography technique provides high selectivity, high resolution and high capacity. High purity can often be achieved in a single step. Large sample volumes can be handled and samples can be applied under conditions that favor specific binding to the ligand. Elution is often performed under gentle conditions which helps preserve bioactivity. The target protein is eluted in a purified and concentrated form by modification of pH, ionic strength, or by introducing a competitive ligand.

Co-NTA Agarose for His-tagged protein purification > - Co-NTA MagBeads His-tagged protein purification > - Disposable columns 0.5mL > and 20mL > - Ni-IDA Agarose for His-tagged protein purification > - Ni-NTA-Agarose for His-tagged protein purification > - Ni-IDA MagBeads His-tagged protein purification > - Ni-NTA MagBeads His-tagged protein purification > - prepacked Ni-IDA Agarose columns

Affinity chromatography is a useful technique for separating proteins in a reversible interaction between the target protein and ligands bound to beads (affinity resin). The interaction can be... read more »
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Affinity Chromatography Purification

Affinity chromatography is a useful technique for separating proteins in a reversible interaction between the target protein and ligands bound to beads (affinity resin). The interaction can be biospecific, for example antibodies binding to protein A, or non-biospecific, for example histidine-tagged proteins binding to metal ions.

The chromatography technique provides high selectivity, high resolution and high capacity. High purity can often be achieved in a single step. Large sample volumes can be handled and samples can be applied under conditions that favor specific binding to the ligand. Elution is often performed under gentle conditions which helps preserve bioactivity. The target protein is eluted in a purified and concentrated form by modification of pH, ionic strength, or by introducing a competitive ligand.

Co-NTA Agarose for His-tagged protein purification > - Co-NTA MagBeads His-tagged protein purification > - Disposable columns 0.5mL > and 20mL > - Ni-IDA Agarose for His-tagged protein purification > - Ni-NTA-Agarose for His-tagged protein purification > - Ni-IDA MagBeads His-tagged protein purification > - Ni-NTA MagBeads His-tagged protein purification > - prepacked Ni-IDA Agarose columns

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FLAG-Peptide - Co-NTA-Agarose Ni-NTA Agarose Ni-IDA Agarose Ni-NTA MagBeads Protein Pre-Packed Empty Spin 3x FLAG Peptide Ni-IDA MagBeads Protein Empty Spin Co-NTA MagBeads
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